ROLE OF 3,5-DIIODO-L-THYRONINE ON THE PITUITARY-THYROID AXIS
- Autori: Giammanco, M.; Marino, A.; LA GUARDIA, M.; DI MAJO, D.
- Anno di pubblicazione: 2018
- Tipologia: Proceedings (TIPOLOGIA NON ATTIVA)
Studies have highlighted that the administration of 3,5-diiodo- L-thyronine to rats fed diets rich in lipids induces a decrease of cholesterol and triglycerides plasma levels and body weight without inducing liver steatosis. On the basis of these observations we carried out some experimental in vivo studies to assess the effects of multiple high doses of T2 on the pituitary thyroid axis of rats fed diet rich in lipids. Fifteen male Wistar rats were divided into three groups of five animals each. The first group (N group) received standard diet, the second group was fed with a high fat diet (HFD group) while the third group (HFDT2 group), was additionally given with T2 intraperitoneally at a dose level of 70 g/100 g of body weight three time a week up to four weeks. At the end of the treatment period blood sample from each animal was collected, centrifuged and the resultant serum was stored at -20°C. The serum concentrations of TSH, T3, T4, ACTH, triglycerides, cholesterol, glucose, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase were then determined at various intervals. In addition, liver of rats was examined by histology order to assess the presence and degree of steatosis. The administration of T2 to rats fed with a high fat diet suppressed TSH secretion (p=0.013) while no steatosis was observed in the liver of these animals. These preliminary data show that multiple administration of high doses of T2 to rats fed diets rich in lipid inhibits TSH secretion and prevents the onset of liver steatosis in these animals.