Confocal microscopy study of the distribution, content and activity of mitochondria during Paracentrotus lividus development
- Autori: MORICI G; AGNELLO M; SPAGNOLO F; ROCCHERI MC; DI LIEGRO CM; RINALDI AM
- Anno di pubblicazione: 2007
- Tipologia: Articolo in rivista (Articolo in rivista)
- Parole Chiave: development; sea urchin; mitochondrial mass; CLSM
- OA Link: http://hdl.handle.net/10447/23691
Abstract
In the present paper we applied confocal microscopy and fluorescence technologies for studying the distribution and the oxidative activity of sea urchin (Paracentrotus lividus) mitochondria during development, by in vivo incubating eggs and embryos with cell-permeant MitoTracker probes. We calculated, by a mathematical model, the intensity values, the variations of intensity, and the variation index of incorporated fluorochromes. Data demonstrate that mitochondrial mass does not change during development, whereas mitochondrial respiration increases. In addition, starting from 16 blastomeres stage, some regions of the embryo contain organelles more active in oxygen consumption.