Study of variability of an endophytic acremonium population in symptomless grapevine
- Autori: Carra, E; Salerno, B; Conigliaro, G; Burruano, S
- Anno di pubblicazione: 2009
- Tipologia: eedings
- Parole Chiave: RAPD PCR;
- OA Link: http://hdl.handle.net/10447/46194
Abstract
The endophytism of genus Acremonium in symptomless grapevines of several cultivars, grown both in pots or in the field, is reported. The no-tillage management of tested grapevines and the lack of chemical treatments facilitated the investigation of the ecological plant-fungus interaction. The isolates were differentiated at the strain level by vegetative compatibility assay and randomly amplified polymorphic DNA (RAPD) analyses, using a set of four oligo-nucleotide primers. The endophytic Acremonium population in the healthy grapevine object of the survey seemed to show a low variability; all the techniques agreed in grouping the Acremonium strains in three different clusters and no relationships were observed among them.