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Expansion of intracellular IFN-g positive lymphocytes during Mycoplasma agalactiae infection in sheep

  • Autori: La Manna, MP; Agnone, A; Villari, S; Puleio, R; Vitale, M; Nicholas, R; Sireci, G; Dieli, F; Loria, GR
  • Anno di pubblicazione: 2011
  • Tipologia: Articolo in rivista (Articolo in rivista)
  • Parole Chiave: Agalactia, Mastitis, T cells, Peripheral blood mononuclear cells, CD4+ cells, CD8+ cells
  • OA Link:


A method to assess the expansion of antigen-specific intracellular IFN-c positive T cell subsets during the infection will be helpful for a better understanding of mycoplasmal infections physiopathology in the sheep. We analysed the percentage of antigen-specific lymphocytes positive for intracellular IFN-c during the infection of sheep with Mycoplasma agalactiae by culturing peripheral blood mononuclear cells of infected or uninfected animals with irradiated M. agalactiae. The expansion of antigen-specific IFN-c positive lymphocytes in infected sheep was initially sustained by CD4+ T cells at day 15 after infection, when antigen specific IgG start to be detectable, followed by CD8/IFN-c double positive cells. cd T-cells were not expanded at any time point analysed. IFNc+ T cells disappear 60 days after infection, suggesting that antigen specific IFNc+ T cells, mainly detected in the early phase of the disease, could be useful to understand the role of cell-mediated immunity during M. agalactiae infection.