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Binding of flunitrazepam to differentiating neurons cultured in a chemically defined, hormone-supplemented medium

  • Autori: Savettieri, G.; Guarneri, R.; Salemi, G.; LA BELLA, V.; Ferraro, D.; Scondotto, S.; Piccoli, F.
  • Anno di pubblicazione: 1990
  • Tipologia: Articolo in rivista (Articolo in rivista)
  • Parole Chiave: Flunitrazepam; GABA; neuronal culture; Biochemistry; Cellular and Molecular Neuroscience
  • OA Link:


[3H]Flunitrazepam (FNZ) binding to cortical neurons from fetal rat brain was investigated in vitro. The use of a synthetic medium specific for neurons made it possible to plot a developmental curve of3H-FNZ binding in an almost pure neuronal culture. Detectable specific binding was present in vitro at time 0 (that is, the 16th gestational day). A progressive increase of binding, due to an increment in the number of recognition sites, was observed on the subsequent days. The affinity of the specific binding sites to3H-FNZ was enhanced by the addition of exogenous GABA, whereas the density was not affected. © 1990 Plenum Publishing Corporation.