VP7 and VP4 Sequence Analyses of Rotavirus Strains From Italian Children With Viraemia and Acute Diarrhoea.
- Autori: Chiappini, E.; Arista, S.; Moriondo, M.; DE GRAZIA, S.; Giammanco, G.; Azzari, C.; Galli, L.; De Martino, M.
- Anno di pubblicazione: 2010
- Tipologia: Articolo in rivista (Articolo in rivista)
- Parole Chiave: rotavirus; children; viremia; sequence analysis
- OA Link: http://hdl.handle.net/10447/42993
Information on genotypes or variants associated with systemic rotavirus infection is lacking. Seven viraemic children with acute rotavirus diarrhoea were evaluated. All faecal strains were genotyped (5 G1P type, 1 G9P, 1 G1-G4P). Only 3 of 7 blood strains were typeable (G1P, G4P8, and G1P[ND]). A discrepancy between faecal and blood VP7 amino acid sequences (Ser-->Asp at position 94) was found in the first child. Two amino acid substitutions (Ile-->Val and Asn-->Ser at position 106 and 113) in the VP4 sequences were demonstrated in the second child. Complete correspondence was found in the third child. All of the observed amino acid substitutions may be involved in rotavirus neutralisation. We speculate that during co-infection by multiple strains, a preferential extraintestinal dissemination of some variants may occur, possibly because of differential viral characteristics.