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GIUSEPPE CALVARUSO

Inhibition of DNA synthesis in chick embryo retinas, in vitro, by a factor from fetal bovine serum

  • Autori: Tesoriere, G.; Vento, R.; Calvaruso, G.; Schiavo, M.; Giuliano, M.; Taibi, G.
  • Anno di pubblicazione: 1989
  • Tipologia: Articolo in rivista (Articolo in rivista)
  • OA Link: http://hdl.handle.net/10447/65746

Abstract

Fetal bovine serum inhibited deoxyribonucleic acid (DNA) synthesis in chick embryo retina explants. The inhibitory activity was precipitated from fetal bovine serum by 45% saturated ammonium sulfate and isolated by means of Sephadex G-100 and Bio-Gel P-60 columns as a peak with an apparent molecular weight of 7000 Da. DNA-inhibiting activity was heat- and acid-stable and was destroyed by dithiothreitol and alkaline treatment. The purified factor inhibited similarly both DNA synthesis and thymidine kinase activity; 50% inhibitory effect was found with 160 ng, 17 h after the addition into the incubation medium.