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GIUSEPPE PIZZOLANTI

In vitro cellular co-colture method to induce immunological tolerance

Abstract

An in vitro method able to modulate peripheral blood mononuclear cells of patients with auto-immune organ-specific diseases is described. The protocol makes auto-reactive lymphocytes tolerogenic through a process of re-education in recognition of self-antigen after co-culture with heterologous and/or autologous fibroblast limbal stem cells.