POSTER SESSIONS

MONDAY SEPTEMBER 23

The lectin pathway

1

SOLUBLE PEPTIDOGLYCAN CAN ACTIVATE THE LECTIN COMPLEMENT PATHWAY

Ma Young Girl1, Zhaong Rong1, Ji-Won Park1, Misao Matsushita2, Teizo Fujita2 and Bok Luel Lee1

317

MASP ACTIVITY IN HUMAN SERUM

Xin Ji, Ying Chen, Thomas F. Lint and Henry Gewurz

2

MANNOSE-BINDING LECTIN INCREASES PHAGOCYTOSIS BY AN ENHANCEMENT OF INTERNALISATION DEPENDENT ON ACTIN REARRANGEMENT

DL Jack1,2, M Lee1, MW Turner2, NJ Klein2, RC Read1

7

FUNCTIONAL CHARACTERIZATION OF THE LECTIN PATHWAY OF COMPLEMENT IN FULLHUMAN SERUM IN RELATION TO MBL GENOTYPE

. A. Roos1, L.H. Bouwman1, J. Munoz1, T.Zuiverloon1, M.E. Wildenberg1, M.C. Faber-Krol1, F.C. Fallaux-van den Houten1, M.G. Tilanus2, C.E. Hack3, and M.R. Daha1.

55

ROLE OF MASP-1AND/OR MASP-3 IN ACTIVATION OF THE LECTIN PATHWAY 

M. Takahashi, M. Matsushita, Y. Endo and T. Fujita, 

71

MANNOSE-BINDING LECTIN DEFICIENCY - REVISITED 

Peter Garred1, Flemming Larsen1, Hans O. Madsen1, Robert B. Sim2 & Claus Koch3 

77

C1s/MASP-2 CHIMERAS: TOOLS TO DETERMINE THE RELATIVE CONTRIBUTIONS OF THE CCP MODULES AND SERINE PROTEASE DOMAIN OF MASP-2 TO ITS HIGHER C4 CLEAVING ACTIVITY 

V. Rossi, F. Teillet, I. Bally, & G.J. Arlaud 

78

L-FICOLIN/P35 BINDS TO MBL-ASSOCIATED SERINE PROTEASES-1 AND -2 WITH HIGH AFFINITY AND COMPETES WITH MBL  

S. Cseh1, L. Vera1, M. Matsushita2 T. Fujita2, G.J. Arlaud1, & N.M. Thielens1

79

ACTIVATION OF MBL-ASSOCIATED PROTEASES-1 AND -2 IS AN AUTOLYTIC PROCESS 

S. Zundel, S. Cseh, M. Lacroix, G.J. Arlaud, & N.M. Thielens 

129

THE MANNOSE-BINDING LECTIN-MASPs SYSTEM:A POTENTIAL LINK BETWEEN COMPLEMENT AND A DEFENSIVE ROLE OF COAGULATION. 

K Hajela *, M. Kojima, J. Ferluga , G. Ambrus, P. Gal, and R. B. Sim 

306

Organ distribution of the murine mannan binding lectins MBL-A and MBL-C.

Wagner S, Lynch N J+, Walter W, Schwaeble W+, Loos M,

307

DIFFERENTIAL EXPRESSION OF MURINE MBL-A AND MBL-C IN B CELLS, DENDRITIC CELLS AND MACROPHAGES BY IMMUNOREGULATORY AND PROINFLAMMATORY CYTOKINES. 

Wagner, Swen, Walter, Wolfgang, and Loos, Michael,

57

NATURAL SUBSTRATES AND INHIBITORS OF MASP-1 AND MASP-2. A STUDY ON RECOMBINANT FRAGMENTS. 

G. Ambrus1, P. Gál1, M. Kojima2, K. Szilágyi1, A. Laich2, Zs. Lõrincz1, B. E. Moffatt2, R. B. Sim2 and P. Závodszky1 

Mechanisms and regulation of activation

149

ENDOTHELIAL CELL PROLIFERATION INDUCED BY SUBLYTIC C5b-9 REQUIRES THE ACTIVATION OF p70S6 KINASE THROUGH A MECHANISM INVOLVING THE G PROTEIN-DEPENDENT ACTIVITY OF PHOSPHATIDYLINOSITOL 3-KINASE

Florin Niculescu*#, Sorana Hila§, Matthew Fosbrink*, Lucian Soane*, Teodora Popescu*, Valentina Shustova.#, Violeta Rus#, Moon L. Shin*, and Horea Rus* 

212

THE SITE OF C4 DEPOSITION UPON COMPLEMENT ACTIVATION VIA THE MBL PATHWAY OR THE CLASSICAL PATHWAY.  

Mette Møller-Kristensen, Steffen Thiel, Annette G. Hansen og Jens C. Jensenius

221

ADSORBED C3 CAN FORM AN INITIATING APW CONVERTASE

Jonas Andersson, Kristina Nilsson Ekdahl, Rolf Larsson, Ulf R. Nilsson, Bo Nilsson

301

A FUNCTIONAL ASSAY FOR THE MBL PATHWAY OF COMPLEMENT ACTIVATION

Steffen Thiel, Libeth Jensen, Annette G. Hansen and Jens Chr. Jensenius

304

CARBOHYDRATE - LIGANDS ON HUMAN TUMOR CELLS TO MANNAN-BINDIGN PROTEIN, MBP

Toshisuke Kawasaki1,2, Tomoaki Nakagawa1,2, Risa Inoue2, Kanako Kamida2, Saori Shibayama2 , Takae Arai1,2 and Nobuko Kawasaki2,3

13

EVIDENCE FOR COMPLEMENT ACTIVATION BY SERUM AMYLOID P COMPONENT (SAP) IN VIVO

Atoosa Familian, Gerard J. van Mierlo, C. Erik Hack

14

RAT C-REACTIVE PROTEIN ACTIVATES THE ENDOGENOUS COMPLEMENT SYSTEM.

Niubel Diaz Padilla a, Wim K. Bleeker a, Yvonne Lubbers a, Gemma M. M. Rigter a, G. van Mierlo a, Mohamed R. Dahab, C. Erik Hack a, 

41

COMPLEMENT-MEDIATED UPTAKE OF OPSONIZED ZYMOSAN PARTICLES BY HUVEC 

H. Langeggen, E. Namork, E. Johnson and G. Hetland 

130

COMPLEMENT RECEPTORS TYPE 1 (CR1, CD35) AND 2 (CR2, CD21) ON HUMAN B CELLS PROMOTE MEMBRANE DEPOSITION OF C3-FRAGMENTS AND MAC AND CONCOMITANT MEMBRANE DEPOLARISATION.  

Claus H. Nielsen1, Morten L. Pedersen2, Wolfgang M. Prodinger3, and R. Graham Q. Leslie2,

137

A STATEGY TO COORDINATE THE COMPLEMENT COMPONENT C1Q ON THE CELLS SURFACE BY A MEMBRANE BOUND PTX3.. 

Eleonora Nucera1, Stefania Militi1, Maria L. Pacello1, Caterina Chiapparino1, Barbara Bottazzi2 Alberto Mantovani2, Alma J. Nauta3, Mohamed R. Daha3, Ragnar Lindstedt1, Ottaviano Serlupi1, Paolo Carminati1, Rita De Santis1 & Giovanni Salvatori1

197

BLOCKADE OF COMPLEMENT RECEPTORS ON FOLLICULAR DENDRITIC CELLS 

W. M. Prodinger, W. Sterlacci, S. Fritz, M. Wurm and L. Kacani 

199

STUDIES OF THE MECHANISM OF DECAY ACCELERATING ACTIVITY FOR C3 CONVERTASES USING CR1-BASED INHIBITORS..  

M Krych-Goldberg, R. Hauhart and J. P. Atkinson.

216

ECTOSOMES EXPRESSING THE COMPLEMENT REGULATORY PROTEINS DAF, MCP AND CR1 ARE RELEASED BY PMN AND BIND C1Q. 

Olivier Gasser, Jürg A. Schifferli 

220

ANTIGEN PRESENTING CELL EXOSOMES ARE PROTECTED FROM COMPLEMENTMEDIATED LYSIS BY EXPRESSION OF CD55 AND CD59.

Aled Clayton*, Claire L. Harris†, Jacquelyn Court*, Malcolm D Mason*, B. Paul Morgan†.

237

THE DISSOCIATION OF C1Q FROM IMMUNE COMPLEXES AND THE REGULATION OF C1 BY THE ACTIVATION OF C3  

C. G. Hester and M.M. Frank

253

PRIMARY HUMAN HEPATOCYTES ARE PROTECTED BY MULTIPLE COMPLEMENT REGULATORY PROTEINS.

J. Halme1, M. Sachse1, H. Vogel1, U. Treichel2, P. Zipfel3, T. Giese1, M. Kirschfink1.

96

ANALYSIS OF COMPLEMENT INHIBITORY CAPACITIES OF THE GPI AND TRANSMEMBRANE FORMS OF MOUSE DAF, MCP, CRRY AND HUMAN sCR1 

Damian Kraus,* Joel M. Guthridge,* Henry C. Marsh Jr.,‡ and V. Michael Holers* 

3

HUMAN NEUTROPHIL PEPTIDE-1 BINDS TO C1Q AND MBL AND INHIBITS ACTIVATION OF BOTH THE CLASSICAL PATHWAY AND THE LECTIN PATHWAY OF COMPLEMENT.

T.H.Ramwadhdoebé, D. van den Ham, P.S. Hiemstra1, J.W. Drijfhout2, M.R. Daha and A. Roos, 

6

FACTOR H BINDS TO AND ACTIVATES PLATELETS

Fariba Vaziri-Sani, Ruxandra Iancu, Jens Hellwage, Peter F. Zipfel, Domniki Papadopoulou, Diana Karpman

8

CCP1-4 OF THE C4b-BINDING PROTEIN _-CHAIN ARE REQUIRED FOR FACTOR I MEDIATED CLEAVAGE OF C3b

Lena Kask, Anna M. Blom and Björn Dahlbäck 

86

A ROLE FOR FACTOR H IN REGULATING CLASSICAL PATHWAY ACTIVATION 

LA Tan, U Kishore, J Ferluga, B-bYu, RB Sim. 

126

TRANSCRIPTIONAL REGULATION OF THE FACTOR H RELATED GENES. 

Rebecca Ormsby*, Jens Hellwage#, Dean Male*, Peter F. Zipfel# and David L. Gordon*. 

107

DIFFERENTIAL ROLES OF DAF, CRRY AND CD59 IN REGULATING ALTERNATIVE AND CLASSICAL PATHWAY COMPLEMENT ACTIVATION ON MURINE ERYTHROCYTES 

T. Miwa1, L. Zhou1, B. Hilliard2, J. D. Lambris2, H. Molina3 and W.-C. Song1

101

ENDOTHELIAL CELLS EXPRESS MEMBRANE-BOUND C7 THAT FUNCTIONS AS A DECOY ACCEPTOR FOR THE ASSEMBLING TERMINAL COMPLEX. 

F. Bossi1, V. Pellis1, P. Macor1, R. Bulla1, R. Würzner2, and F. Tedesco1 

24

A NOVEL MECHANISM FOR COMPLEMENT ACTIVATION ON THE SURFACE OF B CELLS FOLLOWING ANTIGEN BINDING 

. Manderson AP1,2, Botto M2, Walport MJ2, Goodnow CC3, Parish CR1

 Structure and Function

68

Automated large-scale protein structure modelling of individual human CCP-modules 

D. C. Soares1, D. L. Gerloff2 and P. N. Barlow1,2 

315

Localization of Complement Regulatory Domains in Human Factor H.

Rebecca Ormsby#, T. Sakari Jokiranta*, Eleni Giannakis#, Thomas Duthy#, Kim Griggs# and David L. Gordon

157

REGULATION OF FACTOR I COFACTOR ACTIVITY OF C4b-BINDING PROTEIN AND FACTOR H BY ZINC 

Anna M. Blom, Björn Dahlbäck and Andreas Hillarp 

178

STRUCTURAL AND FUNCTIONAL CHARACTERISATION OF RECOMBINANT BOVINE CONGLUTININ COMPRISING THE α-HELICAL NECK REGION AND CARBOHYDRATE RECOGNITION DOMAIN 

Alpana Prasad1, Ren Jinshan3, Alister Dodds2, Kenneth BM Reid2 

16

C1r-LIKE PROTEIN (C1r-LP): A NOVEL HUMAN COMPLEMENT-RELATED PROTEIN. 

C.Ligoudistianou, G. Garnier, A. Circolo, J.E. Volanakis. 

92

ISOLATION AND CHARACTERIZATION OF A NOVEL RAT FACTOR H RELATED PROTEIN THAT IS UPREGULATED IN GLOMERULI UNDER COMPLEMENT ATTACK  

Guohui Ren, Mona Doshi, Bradley K. Hack, Jessy J. Alexander, and Richard J. Quigg

109

CHARACTERIZATION OF MEMBRANE COFACTOR PROTEIN (MCP, CD46) ON SPERMATOZOA: ROLE FOR A COMPLEMENT REGULATORY PROTEIN IN FERTILIZATION.  

1Rebecca C. Riley, 2Pamela Tannenbaum, 2Dave Abbott, 1John P. Atkinson

113

FUNCTIONAL CHARACTERIZATION OF HUMAN FACTOR H-RELATED PROTEIN 5 (FHR-5) REVEALS HEPARIN BINDING AND NOVEL COFACTOR ACTIVITY.  

Thomas G. Duthy1, Jennifer L. McRae2, Kim M. Griggs1, Brendan F. Murphy2 and David L. Gordon1.

17

OVERCOMING COMPLEMENT RESISTANCE OF HUMAN CARCINOMA CELLS. . 

Natalie Donin, Katrin Jurianz2, Lea Ziporen, Stefan Schultz2, Michael Kirschfink2 and Zvi Fishelson

102

TREATMENT OF MCF-7 BREAST CANCER CELLS WITH TAMOXIFEN AND FASLODEX (ICI 182780) AUGMENTS COMPLEMENT RESISTANCE BY INCREASING THE EXPRESSION OF THE COMPLEMENT REGULATORS CD55 AND CD59. 

Neil K Rushmere*, Denise M Barrow†, Stephen Tomlinson‡, Robert I Nicholson† & B. Paul Morgan.* 

152

CELL PROLIFERATION INDUCED BY C5b-9 IS MEDIATED BY RGC-32 VIA ACTIVATION OF CDC2 KINASE. 

Tudor Badea*, Florin Niculescu*§, Lucian Soane*, Matthew Fosbrink*,Hila Sorana+, Violeta Rus§, Moon L. Shin* and Horea Rus*+, 

165

CD59 EXPRESSION PROMOTES EL4 TUMOR GROWTH AND METASTASES IN SYNGENEIC C57BL/6 MICE.  

M. Imai1, F. Hong2, G. Ross2 and S. Tomlinson1.

166

EXPRESSION OF H-FICOLIN/HAKATA ANTIGEN, AND MANNOSE-BINDING LECTINASSOCIATED SERINE PROTEASE-1, AND -3 BY HUMAN GLIOMA CELL LINE T98G. 

Mikio Kuraya1, Misao Matsushita1, Yuichi Endo1, Steffen Thiel2, and Teizo Fujita1.

172

IDENTIFICATION OF HEPARIN-BINDING SITES OF C1 INHIBITOR.  

Shenghe Cai and Alvin E. Davis III.

185

ELASTASE FROM ACTIVATED HUMAN NEUTROPHILS ACTIVATES PROCARBOXYPEPTIDASE R 

Takeshi Kawamura, Noriko Okada* and Hidechika Okada 

190

MUTATIONAL ANALYSIS OF HUMAN CR2/CD21 BASED ON THE DETERMINATION OF ITS X-RAY CRYSTALLOGRAPHIC STRUCTURE IN COMPLEX WITH C3D. 

Jonathan Hannan1, Kendra Young1, Gerda Szakonyi2, Rengasamy Asokan1, Xiaojiang Chen2, and V. Michael Holers1 

196

DECAY ACCELERATION OF THE AP C3 CONVERTASE BY COMPLEMENT RECEPTOR 1. 

L.Mitchell1, X. Wang1, M. Krych-Goldberg1, M. Dybvig1, L. Kuttner-Kondo2, M. E. Medof2, J. P. Atkinson1, and D. Hourcade1; 

202

STRUCTURAL AND FUNCTIONAL ALTERATIONS IN THE SLE1C ALLELE OF CR2 CAN BE ATTRIBUTED TO A SINGLE NUCLEOTIDE POLYMORPHISM IN SCR1. 

Susan A. Boackle1, Julie J. Kachinski1, Karla D. Kenyon1, David R. Karp2, Laurence Morel3. 

226

BINDING OF ANAPHYLATOXINS TO IMMUNOGLOBULIN F(ab)'2 FRAGMENTS: A SURFACE PLASMON RESONANCE STUDY

Lajos Baranyi1 and Milan Basta2,

241

CRYSTALLIZATION OF COBRA VENOM FACTOR AND PRELIMINARY CRYSTALLOGRAPHIC ANALYSIS.

Sujata Sharma, Talat Jabeen, Rajendra Kumar Singh, Reinhard Bredehorst, Carl-Wilhelm Vogel, Christian Betzel, and Tej P. Singh.

247

EXPRESSION OF COMPLEMENT FACTOR H-RELATED MOLECULES ON THE CELL SURFACE OF HUMAN URINARY EPITHELIAL CELLS

Li K, Zhou W, Sacks SH, Sheerin NS.

252

MATURATION DEPENDENT EXPRESSION OF cC1q-R AND gC1q-R ON HUMAN DENDRITIC CELLS.

Z. Vegh-Goyarts, E. Goyarts*, K. Rozengarten, A. Mazumder, and B. Ghebrehiwet.

258

TYPE A DOMAIN SITES IN C2a HOMOLOGOUS TO THOSE IN Bb ARE INVOLVED IN C4b2a DECAY ACCELERATION.

L. Kuttner-Kondo,1 M. Dybvig,2 L. Mitchell,2 J. P. Atkinson,2 M. E. Medof,1 and D. Hourcade,2

259

DECAY ACCELERATING FACTOR CCP2-3 IS SUFFICIENT TO ACCELERATE DECAY OF THE CLASSICAL PATHWAY C3 CONVERTASE.

L. Kuttner-Kondo,1 F. Lin,1 D. Hourcade2, and M. E. Medof.

263

CD93 MODULATES INTEGRIN AFINITY AND MOTILITY OF HUMAN NEUTROPHILS

Ionita Ghiran*, Lloyd B. Klickstein† and Anne Nicholson-Weller*

264

STRUCTURE OF THE COBRA VENOM FACTOR (CVF) GENE AND COMPARISON WITH THE HUMAN C3 GENE.

Holger Bammert, Bärbel Kunze, Yan Li, David C. Fritzinger, Reinhard Bredehorst, and Carl-Wilhelm Vogel.

267

IDENTIFICATION OF THE DOMAIN AND AMINO ACIDS NEEDED FOR FACTOR H DIMERIZATION.

T. Sakari Jokiranta*, Rebecca Ormsby#, David L. Gordon#, Peter F. Zipfel+, Seppo Meri*, and Jens Hellwage+.

270

SUBLYTIC TERMINAL COMPLEMENT COMPLEX INDUCED C-JUN N TERMINAL KINASE ACTIVITY MEDIATED PO MYELIN GENE STABILITY.

S. Hila and C.L. Koski,

279

CHARACTERISATION OF THE MOUSE ANALOGUES OF CD59 USING NOVEL MONOCLONAL ANTIBODIES: TISSUE DISTRIBUTION AND FUNCTIONAL COMPARISON.

Claire L. Harris, S. Melanie Hanna, Dewi S. Holt, Kevin J. Marchbank and B. Paul Morgan.

282

HIGH-AFFINITY C5 CONVERTASES OF THE CLASSICAL PATHWAY OF COMPLEMENT

Nenoo Rawal and Michael K. Pangburn

283

HIGH RESOLUTION STRUCTURE OF THE C345C/NTR DOMAIN OF HUMAN C5..

R. T Sareth, G.-P. Zhou, C.-T. Thai, and N. Assa-Munt  Ogata, S.

285

ULTRACENTRIFUGATION OF THE COMPLEX FORMED BETWEEN CR2 SCR-1/2 AND C3D SHOWS THAT SCR-1 IS EXTENDED AWAY FROM SCR-2 IN THE COMPLEX

Hannah E. Gilbert, Jonathan Hannan*, V. Michael Holers* and Stephen J. Perkins

296

HUMAN HEPATOCYTES EXPRESS THE ANAPHYLATOXIN C5A RECEPTOR (CD88) ..

M Sachse1, J. Halme1, F. Autschbach2, E. Klar 3, T. Giese1, and M. Kirschfink1.

169

XENOREACTIVE ANTI-GAL ANTIBODIES ACTIVATE BOTH CLASSICAL AND ALTERNATIVE COMPLEMENT PATHWAYS

AJ Walpen1, PJ Mohacsi1, A Roos2, MR Daha2, R Rieben1

21

THE CRYSTAL STRUCTURE OF HUMAN COMPLEMENT PROTEIN C8γ PROVIDES NEW INSIGHT INTO ITS FUNCTION 

. J.M Sodetz, E. Ortlund, C.L. Parker, S.F. Schreck and L. Lebioda,

25

C1QRP INTERACTS WITH THE PDZ DOMAIN CONTAINING PROTEIN GIPC VIA A CLASS 1 PDZ BINDING MOTIF 

Suzanne S. Bohlson, Mingyu Zhang, Christopher Ortiz and Andrea J. Tenner 

37

THE CRYSTAL STRUCTURE OF gC1q-R/p33: LOCALIZATION AND PREDICTION OF LIGANDBINDING AND CELL-BINDING DOMAINS.  

Jolyon Jesty, Ellinor I.B. Peerschke, and Berhane Ghebrehiwet.

52

COMPUTATIONAL STUDIES TO ASSESS THE NATURE OF C3d-CR2 ASSOCIATION 

Dimitrios Morikis* and John D. Lambris† 

63

SOLUTION STRUCTURE AND DYNAMICS OF CENTRAL CCP MODULE PAIR OF DECAY ACCELERATING FACTOR (DAF).  

S. Uhrinova1, F. Lin2, D. Uhrin1, G. Ball1, M. E. Medof2 and P. N. Barlow1.

64

RESIDUE GLN339, AT THE INTERFACE BETWEEN THE CCP1 AND CCP2 MODULES OF C1s, IS A KEY ELEMENT OF C4 RECOGNITION 

I. Bally, V. Rossi., N.M. Thielens, & G.J. Arlaud 

65

CRYSTAL STRUCTURE OF THE ACTIVE CATALYTIC DOMAIN OF HUMAN C1r  

M. Budayova-Spano1, W. Grabarse2, N.M. Thielens1, H. Hillen3, M. Lacroix1, M. Schmidt3, J.C. Fontecilla-Camps1, G.J. Arlaud1, & C. Gaboriaud1

75

CRYSTAL STRUCTURE OF THE ZYMOGEN CATALYTIC DOMAIN OF HUMAN C1r REVEALS THAT DISRUPTION OF THE C1r HOMODIMER IS REQUIRED TO TRIGGER ACTIVATION OF THE C1 COMPLEX 

M. Budayova-Spano, M. Lacroix, N.M. Thielens, G.J. Arlaud, J.C. Fontecilla-Camps, & C. Gaboriaud. 

80

MUTATIONAL ANALYSES OF RECOMBINANT GLOBULAR HEAD REGIONS SUGGEST CENTRAL ROLE FOR ARGININE AND HISTIDINE RESIDUES IN C1Q-IMMUNOGLOBULIN INTERACTIONS 

M. Kojouharova1, M. Gadjeva2, I. Tsacheva1, A. Zlatarova1, L. Roumenina1, K. B. M. Reid3 and U. Kishore 

82

BIOPHYSICAL STUDIES ON THE COMPLEMENT REGULATOR MEMBRANE COFACTOR PROTEIN 

Herbert A. P., O’Leary, J. & Barlow P. N. 

88

EXPRESSION, STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF THE NETRIN DOMAINS OF COMPLEMENT C3 AND C4 

. John J. Wang and David E. Isenman,

93

NMR STRUCTURE OF THE FUNCTIONAL C3B/C4B BINDING SITE OF COMPLEMENT RECEPTOR TYPE 1 (CR1 OR CD35) AND IDENTIFICATION OF RESIDUES INVOLVED IN THE BINDING INTERACTION 

R.L.Mallin, B.O.Smith, M.Krych-Goldberg*, X.Wang*, R. E. Hauhart, K. Bromek, D.Uhrin, J.P.Atkinson*, P.N. Barlow 

94

THE INTERACTION OF ISOTYPIC FORMS OF HUMAN C4b AND C4d FRAGMENTS WITH COMPLEMENT RECEPTOR 1 AS ASSESSED BY SURFACE PLASMON RESONANCE.  

Liliana Clemenza and David E. Isenman,

131

A MOLECULAR MODEL FOR HUMAN FACTOR B BY CONSTRAINED SCATTERING MODELLING 

Julian T. Eaton, Dean Chamberlain, Justin Hinshelwood, Robert B. Sim* and Stephen J. Perkins 

145

ELECTROMAGNETIC FIELDS CAN MODIFY CELLULAR LYSIS INDUCED BY COMPLEMENT* 

Maria Brai1, Antonio Bartolotta2, Liliana Clemenza3, Francesca Damiani1, Carmela La Mendola3, Franco Palla4, Gabriella Palombo3. 

148

RAT COMPLEMENT FACTOR H: MOLECULAR CLONING, SEQUENCING AND QUANTIFICATION WITH A NEWLY ESTABLISHED ENZYME-LINKED IMMUNOSORBENT ASSAY 

T.Demberg, B. Pollok-Kopp, D. Gerke, O. Götze and G. Schlaf,

158

STRUCTURE OF C3b2-IgG COMPLEXES 

Emiliana Jelezarova, Alexander Luginbuehl, and Hans U. Lutz 

164

THE C4b-BINDING PROTEIN _-CHAIN: INFLUENCE OF CCP2 ON BINDING OF PROTEIN S AND DEVELOPMENT OF TWO NEW MONOCLONAL ANTIBODIES 

Webb J.H., Villoutreix B.O., Dahlbäck B. and Blom A.M.

 Evolution and Ontogeny of Complement

312

CLONING AND CHARACTERIZATION OF RAT COMPLEMENT COMPONENT 6 (C6)

Deepak Bhole, Ellen Gormley, Gregory L. Stahl

313

Parameters of the innate complement system of cod (Gadus morhua L.) and halibut (Hippoglossus hippoglossus L.)”.

Lange, S.*,  Dodds, A.W.+ & Magnadóttir, B.*

198

TRANS-SPECIES POLYGENIC VARIATION OF COMPLEMENT C4 AND RCCX MODULAR STRUCTURES IN RHESUS MACAQUE (MACACA MULATTA) AND HUMANS.  

N Longman- Jacobsen, EK Chung, Y Yang, KR Clark, PR Johnson and CY Yu.

229

MOLECULAR CLONING OF COMPLEMENT C7 AND C8â GENES OF RAINBOW TROUT

Alexandra Kazantzi1, Georgia Sfyroera1,2, John D. Lambris2, Ioannis K. Zarkadis1

262

ANCIENT ORIGIN OF MANNOSE-BINDING LECTIN-ASSOCIATED SERINE PROTEASE (MASP)-1/3 GENE

T.Fujita1, M. Nonaka2, H. Saiga3, Y. Kakinuma1, M. Takahashi1, M. Matsushita1 and Y. Endo1

265

COBRA VENOM FACTOR IS A MEMBER OF A MULTI-COPY GENE FAMILY IN COBRA.

Holger Bammert, David C. Fritzinger, Reinhard Bredehorst, and Carl-Wilhelm Vogel.

275

MOLECULAR CLONING OF MANNOSE- BINDING LECTIN GENES OF RAINBOW TROUT

Konstantina Nikolakopoulou, Ioannis K. Zarkadis

277

STRUCTURAL AND FUNCTIONAL DIVERSITY OF FACTOR B/C2-ISOTYPES IN THE COMMON CARP.

Miki Nakao, Makiko Nakahara, Momoe Matsumoto, Yoko Kato, Kazuhiro Fujiki and Tomoki Yano.

278

CLONING OF TWO FACTOR I-ISOTYPES WITH DISTINCT DOMAIN ORGANIZATIONS FROM THE COMMON CARP

. Miki Nakao*, Satomi Hisamatsu*, Makiko Nakahara*, Yoko Kato*, Sylvia L.  Smith† and Tomoki Yano*.

292

DIFFERENCES BETWEEN HUMAN AND MACACA MULATTA CR1 GENE PROMOTER REGIONS AND HUMAN OR MACACA MULATTA ALLELE POLYMORPHISMS.

A. KISSERLI, B. DONVITO, A. GIMENEZ, N. GODIN, V. DURET, M. TONYE LIBYH, F. PHILBERT, B. REVEIL, T. TABARY and J.H.M. COHEN. 

297

MULTIPLE FACTOR I AND TWO FACTOR B ISOFORMS IN THE NURSE SHARK (GINGLYMOSTOMA CIRRATUM).

Dong-Ho Shin1, Barbara M.Webb1, Miki Nakao2 and Sylvia L Smith1,3.

298

EVOLUTION OF C3A ANAPHYLATOXINS AND THEIR ROLE IN INFLAMMATION: STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF THREE DISTINCT C3A ANAPHYLATOXINS IN RAINBOW TROUT.

Hove-Madsen L.1, Parra D. 2, Balasch J.C.1, Liu Q.H. 3, Boshra, H. 2, Freedman B.D. 3, Tort Ll.1, Sunyer J.O.2

299

INFERRING THE EVOLUTION OF THE CLASSICAL PATHWAY THROUGH THE STUDY OF FISH COMPLEMENT

Boshra, H., Sunyer, J.O.

300

EVOLUTION OF COMPLEMENT RECEPTORS: CHARACTERIZATION OF C3B AND C3D RECEPTORS IN FISH

Andy Gelman, David Parra, Nina Bosch and J. Oriol Sunyer.

67

HIGH DEGREE C3 GENE AMPLIFICATION IN A BONY FISH, MEDAKA 

M. Nonaka, D. Umetsu, and M. Y. Matsuo 

111

CONCENTRATIONS OF COMPLEMENT REGULATORY PROTEINS IN NEONATES. ONTOGENY OF VITRONECTIN IN HEALTHY CHILDREN.  

1Ferreira de Paula, P.; 2Latorre, M.R.D.O.; 3Roxo Jr, P.; 3Ferriani, V.P.L.; 4Nudelman, V. and 1Isaac, L.

115

MOLECULAR CLONING AND CHARACTERIZATION OF NOVEL FICOLINS FROM XENOPUS LAEVIS

Kakinuma Y, Endo Y, Takahashi M, Matsushita M and Fujita T 

117

ANCIENT ORIGINS FOR THE C3-LIKE ANCESTOR OF COMPLEMENT COMPONENTS C3/C4/C5 

L.J. Dishaw, C.H. Bigger, and S.L. Smith, 

120

CHARACTERIZATION OF C1q HOMOLOGUE IN LAMPREY 

Misao Matsushita, Akiko Nozawa, Yuichi Endo, Munehiro Nakata*‡, Naoya Kojima*‡, Tsuguo Mizuochi*‡ and Teizo Fujita 

123

STRUCTURE, EXPRESSION AND GENOMIC ORGANIZATION OF SIX COMPLEMENT GENES OF AN ASCIDIAN, CIONA INTESTINALIS 

Fumiko Yoshizaki and Masaru Nonaka 

125

EVOLUTION OF COMPLEMENT REGULATORS: CLONING AND CHARACTERIZATION OF ASCIDIAN RCA CANDIDATES 

. Seita Miyazawa and Masaru Nonaka.

167

THE ASCIDIAN CIONA INTESTINALIS (UROCHORDATA) AS A MODEL TO STUDY EXPRESSION PATTERNS OF COMPLEMENT GENES DURING EMBRYO DEVELOPMENT  

Pinto MR*, Yoshizaki F°, Kimura Y^, Marino R*, Melillo D*, De Santis R*, Nonaka M°, Lambris JD^

WEDNESDAY, SEPTEMBER 25

Genetics and Regulation of Synthesis

29

HUMAN IMMATURE DENDRITIC CELLS ARE A MAJOR SOURCE OF C1Q PRODUCTION. 

Giuseppe Castellano, Moh Daha, Anja Roos, Andrea Woltman, *Francesco Paolo Schena, Cees van Kooten. 

54

COMPLEMENT PROTEIN C1q BINDS TO ANIONIC MEMBRANES: A POTENTIAL ROLE FOR C1q IN REMOVAL OF AGED RED BLOOD CELLS.  

Amanda J Bradley, Elisabeth Maurer-Spurej, Donald E Brooks, and Dana V Devine.

83

COMPLEMENT RECEPTORS CR3 (CD11b/CD18) AND CR4 (CD11c/CD18) EXPRESSED BY DENDRITIC CELLS DO NOT BIND COMPLEMENT FRAGMENT iC3b. 

Preynat-Seauve O. *†, Richard Marie J. *, Jourdan G. *, Favrot M. C. *, Marche P. † and Villiers C. † 

87

ROLE OF COMPLEMENT IN CLEARANCE OF RED CELLS IN AUTOIMMUNE HEMOLYTIC ANEMIA. 

Feng Lin and M. Edward Medof. 

168

DUPLICATE GENES FOR MURINE C1r AND C1s GENERATE ALTERNATIVE ISOMORPHS IN THE LIVER AND THE MALE REPRODUCTIVE SYSTEM.

G. Garnier, A. Circolo, Y. Xu, and J. E. Volanakis.

232

PROGRESSIVE MATURATION OF CR1 DENSITY ON ERYTHROCYTES IN CHILDHOOD

B. DONVITO1, A. ADOCHITEI1, M. TONYE LIBYH1, A. KISSERLI1, A. GIMENEZ1, N. GODIN1, V. DURET1, B. REVEIL1, F. PHILBERT1, Th. TABARY1, J CORNILLET2, and J.H.M. COHEN1.

56

FUNCTIONAL ANALYSIS OF THE CR2/CD21 PROMOTER: IMPORTANCE OF A CELL TYPE SPECIFIC REPRESSOR BINDING E2A PROTEINS.. 

Daniela Ulgiati1, James S. Dods1, Lawrence J. Abraham1 and V.M. Holers2

174

THE ROLE OF PHOSPHATASES AND THE PROTEASOME IN SUPPRESSION OF IFN-ã-MEDIATED STAT1 IN REGULATION OF C1 INHIBITOR SYNTHESIS.  

Dongxu Liu, Kamyar Zahedi, Anne E. Prada, Jennifer Scafidi, and Alvin E. Davis III.

192

YIN YANG 1, OCT1, AND NFAT-4 FORM REPEATING, CYCLOSPORIN-SENSITIVE REGULATORY MODULES WITHIN THE MURINE CD21 INTRONIC CONTROL REGION 

Mark D. Zabel1, Wells Wheeler, Ann B. Sweeney, Janis J. Weis and John H. Weis 

206

FURTHER STUDY ON FACTOR D SYNTHESIS BY NORMAL HUMAN HEPATOCYTES 

Etsuko Kitano, Yasuo Fukumori* and Hajime Kitamura, 

209

C1q SYNTHESIS IN THE BRAINS OF S-_-CHLOROHYDRIN TREATED RATS  

Nicholas J. Lynch, Christopher J. Guérin*, Silke Roscher, Colin Willis‡, Christopher C. Nolan‡, David E. Ray‡ and Wilhelm J. Schwaeble

286

EXTRA-HEPATIC EXPRESSION OF MANNOSE-BINDING LECTIN.

Jeanette Seyfarth, Hans O. Madsen and Peter Garred.

287

THE MOLECULAR MECHANISMS BEHIND INACTIVATION OF THE HUMAN MBL1 GENE.

Jeanette Seyfarth, Peter Garred and Hans O. Madsen.

239

HIGH FREQUENCY OF THE GENOTYPE OF LOW CR1 EXPRESSION ON E AND VERY LOW CR1 DENSITY ON ERYTHROCYTES IN AN ETHNIC MINORITY FROM NORTH EAST CAMBODIA  

B. DONVITO1, A. KISSERLI1, N. GODIN1, A. GIMENEZ1, M. TONYE LIBYH1, V. DURET1, B. REVEIL1, F. PHILBERT1, P. LEMAIRE2, T . TABARY1, D. TOUBAS2, M. GRILLERES1, and J.H.M.COHEN1

244

GENETIC ANALYSIS OF FACTOR H IN A COHORT OF PATIENTS PRESENTING WITH ATYPICAL HUS/TTP: SEVEN NEW MOLECULAR CHARACTERIZATIONS LEADING TO A FACTOR H DEFICIENCY AND LACK OF HOT SPOT FOR MUTATION IN THE SCR 20 AND 19.

M.-A. Dragon-Durey 1,2, V. Frémeaux-Bacchi 1,2, C. Loirat 3, P. Coppo 4, J. Blouin 1, M. Trendelenburg 5, A. Oener 6. W.H. Fridman 1 and L. Weiss 2.

250

FOUR RECURRENT MUTATIONS AND 21 NOVEL MUTATIONS/ SMALL DELETIONS IN THE C1 INHIBITOR GENE

V. Frémeaux-Bacchi 1, M- A. Dragon –Durey 1, J. Blouin 1, J. Laurent 2 and W. H . Fridman1

255

STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF THE R1210C FACTOR H MUTATION ASSOCIATED TO ATYPICAL HEMOLYTIC UREMIC SYNDROME.

Huarte O, Pérez-Caballero D, Simckes A M, Goicoechea E, Sánchez-Corral P and Rodríguez de Córdoba S.

288

MOLECULAR MODELLING OF MUTATIONS IN THE C-TERMINAL DOMAINS OF HUMAN COMPLEMENT FACTOR H: A NEW INSIGHT INTO HAEMOLYTIC URAEMIC SYNDROME

Stephen J. Perkins1 and Timothy H.J. Goodship2,

42

GENETIC DIVERSITY AND SOPHISTICATION OF COMPLEMENT C4A AND C4B AND RP-C4-CYP21- TNX MODULES IN THE HUMAN MAJOR HISTOCOMPATIBILITY COMPLEX (MHC). 

EK Chung1, Y Yang1, B Zhou1, M Lokki 2, CA Blanchong 1, KN Jones1, GC Higgins1, RM Rennebohm1 and CY Yu1. 

140

EBNA2 TARGETS THE HUMAN COMPLEMENT RECEPTOR 2 (CR2) PROMOTER THROUGH HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN D (hnRNP D), AND ENHANCES PROMOTER ACTIVITY.  

M. Tolnay, L.A. Vereshchagina and G.C. Tsokos.

28

TRANSCRIPTIONAL REGULATION OF THE HUMAN C3A-RECEPTOR: ETS-1 AS ONE IMPORTANT FACTOR 

Schaefer M., Konrad S., Sohns B., Hetfeld B., Rheinheimer C., Bautsch W. and Klos A. 

Complement in therapy

22

THE ALTERNATIVE COMPLEMENT PATHWAY IN ISCHEMIA REPERFUSION  

INJURY Gregory L. Stahl*, Hui Zhao*, Michael Fung†, and Yuanyuan Xu‡.

26

A C3a RECEPTOR ANTAGONIST REDUCES INTESTINAL ISCHEMIA/REPERFUSION INJURY IN RATS

L.M. Proctor*, T.V. Arumugam*, R.C. Reid†, D.P. Fairlie†, I.A. Shiels*, S.M. Taylor*, 

30

ATTENUATION OF ARTIFICIAL SURFACE-INDUCED LEUKOCYTE ACTIVATION BY BLOCKING C2, FACTOR D, C5 AND THE C5a RECEPTOR 

K. T. Lappegård1,2, T. Hvassing1, M. Fung3, J. D. Lambris4, J. Riesenfeld5, T. E. Mollnes1. 

31

THE C5A FRAGMENT OF C5 IS CRITICAL FOR THE MESENTERIC ISCHEMIA/REPERFUSIONINDUCED LOCAL AND REMOTE ORGAN INJURY 

. Sherry D. Fleming1,3, John D. Lambris2, Terez Shea-Donohue3, George C. Tsokos1,3

33

HIERARCHICAL ROLE OF COMPLEMENT, NEUTROPHILS AND P-SELECTIN IN THE SETTING OF RENAL ISCHEMIA/REPERFUSION INJURY  

C.A.Farrar, W.Zhou and S.H.Sacks.

35

ASSESSMENT OF AN “ENDOTHELIAL CELL PROTECTANT” IN XENOTRANSPLANTATION MODELS: EFFECT OF LOW MOLECULAR WEIGHT DEXTRAN SULFATE  

T. Laumonier1; P.J. Mohacsi1; E.Y. Korchagina2; N.V. Bovin2; and R. Rieben1

36

PROTECTION OF INTESTINAL NEURAL TISSUE AGAINST COMPLEMENT MEDIATED INJURY BY CD55 EXPRESSION ON SURROUNDING ELASTIC FIBERS. 

K.A. Gelderman, C.F.M. Sier, H.J.M.A.A. Zijlmans, M.J. Vonk, A. Gorter 

38

HUMAN C1 ESTERASE INHIBITOR ATTENUATES MURINE MESENTERIC ISCHEMIA/ REPERFUSION INDUCED LOCAL ORGAN INJURY  

Georg Karpel-Massler*,†, Sherry D. Fleming*, Michael Kirschfink†, George C. Tsokos*.

61

COMPLEMENT C3A RECEPTOR: A NEWLY IDENTIFIED INNATE IMMUNE ENDOCRINE REGULATORY PATHWAY INVOLVED IN CONTROL OF INFLAMMATORY RESPONSES. 

Karen Francis1,2, B. Mary Lewis2, Hiroyasu Akatsu3, Peter Monk4, Maurice F. Scanlon2, B. Paul Morgan1, Jack Ham2 and Philippe Gasque1. 

74

REGULATION OF THE PROMOTER OF HUMAN PROCATHEPSIN-L GENE, A PROTEINASE WHICH CLEAVES HUMAN C3, THE THIRD COMPONENT OF COMPLEMENT AND CONFERS HIGH TUMORIGENIC AND METASTATIC PROPERTIES TO HUMAN MELANOMA CELLS.  

Didier JEAN, Nathalie GUILLAUME and Raymond FRADE.

76

A NON-TRANSGENIC STRATEGY FOR TISSUE ENGINEERING FOR THE MODULATION OF TRANSPLANT ISCHEMIA / REPERFUSION INJURY. 

. M.E Jones1, J.R. Pratt1, I. Dodd2, R.A.G. Smith2 & S.H. Sacks11.

84

NOVEL COMPLEMENT INHIBITORS FOR THE PREVENTION OF ANTIBODY MEDIATED COMPLEMENT ACTIVATION 

A.J. Walpen1, T. Laumonier1, P.J. Mohacsi1, E.Y. Korchagina2, N.V. Bovin2, R. Rieben1 

99

COMPLEMENT INHIBITION AMELIORATES DISEASE IN LUPUS CEREBRITIS 

Jessy J.Alexander, Lihua Bao, V. Michael Holers and Richard J. Quigg 

108

COMPLEMENT INHIBITION WITH CRRY PREVENTS DEVELOPMENT OF LUPUS NEPHRITIS 

Lihua Bao, Jonathan Rakstang, V. Michael Holers and Richard J. Quigg 

134

C1Q-TAILS ACTIVATE P38 MAPK AND APOPTOSIS IN FIBROBLASTS.  

Sandra Bordin, Douglas Whitfield.

142

THE SYNTHETIC PEPTIDE DX88 BINDS TO ENDOTHELIAL CELLS IN VITRO AND RETAINES THE INHIBITORY ACTIVY ON KALLIKREIN.. 

E. Rossi, D. Maiocchi, M.Cicardi, A. Williams§, L.Bergamaschini

146

HEPARIN PREVENTS INFLAMMATORY AND CYTOTOXIC ACTIVITY OF AMYLOID-b IN VITRO.

L.Bergamaschini, E. Rossi, A. Zucchella, C. Donarini, C. Vergani,*M. Di Staso, *M.G. De Simoni. 

147

PROTECTIVE EFFECTS BY C5AR ANTAGONIST (C5ARA) DURING EXPERIMENTAL SEPSIS  

M Huber-Lang, V Sarma, E Younkin, SR McGuire, KT Lu, TA Neff, RF Guo, F Gebhard§, J Lambris#, PA Ward

150

EFFECTS OF DAF AND C1 ESTERASE INHIBITOR (C1-INH) ON XENOGENEIC  

CELL Daisuke Fukuta, Shuji Miyagawa, Mako Yamada, Katsuyoshi Matsunami, Akio Shirasu, Hiroyuki Hattori and Ryota Shirakura.

161

EXCESSIVE COMPLEMENT ACTIVATION AND IMPAIRED INNATE IMMUNITY DURING SEPSIS. 

P.A. Ward, M.S. Huber-Lang, V. Sarma, N.C. Riedemann, F.S. Zetoune, E.M. Younkin, T.A.

176

REVERSAL OF THE INCREASED VASCULAR PERMEABILITY IN C1 INHIBITOR DEFICIENT MICE: THERAPEUTIC APPROACHES.  

Eun D. Han*, Emanuela Pappalardo§, Jennifer Scafidi*, and Alvin E. Davis III*.

180

RECOMBINANT HUMAN C1INHIBITOR: PRECLINICAL DEVELOPMENT 

Bertjan Ziere, Ellen Schenk, Harrie van Veen, Jan Nuijens and Rene Verdonk 

181

A PHASE I STUDY OF RECOMBINANT HUMAN C1 INHIBITOR IN ASYMPTOMATIC PATIENTS WITH HEREDITARY ANGIOEDEMA - HAE 

J Nuijens1, M van Doorn2, T van Dam1, K Burggraaf2, M Levi3, E Hack4 and A Cohen2 

182

COMPLEMENT REGULATOR-Fc FUSION PROTEINS AS PRODRUGS FOR EFFICIENT DELIVERY OF ACTIVE REGULATORS TO SITES OF DISEASE. 

Claire L. Harris, Clare E.Hughes, Bruce Caterson, B. Paul Morgan. 

188

INTERACTION OF POLY(2-ACRYLAMIDO 2-METHYLPROPANE SULFONATE)-GRAFTED POLYSTYRENE BEADS WITH SERUM COMPLEMENT SYSTEM 

Yoshinobu MURAKAMI1, Hiroo IWATA1*, Etsuko KITANO2, Hajime KITAMURA2 and Yoshito IKADA1,3 

189

POLY (ACRYLIC ACID)-GRAFTED POLYSTYRENE BEADS ARE AVAILABLE FOR THE FACTOR D MOLECULES ADSORBENT 

Yoshinobu MURAKAMI1, Etsuko KITANO2, Hajime KITAMURA2, and Hiroo IWATA1 

191

BIOLOGICAL RESPONSES OF OVARIAN CARCINOMA CELLS TO ANTI-HER-2/NEU HUMANIZED MONOCLONAL TRASTUZUMAB IN VITRO 

L. Bjørge1,2,3, O. Vintermyr4, R. El Attal1, T. Skeie Jensen2, R. Würzner1, M.P. Dierich1, O.E. Iversen2, S. Meri5, C. Marth6 , H. Stoiber1 

195

COMPLEMENT AS A KEY MEDIATOR OF THE ANTITUMOR EFFECT OF PHOTODYNAMIC THERAPY.  

Mladen Korbelik, Ivana Cecic and Jinghai Sun,

205

THE SYNTHETIC KUNITZ DOMAIN PROTEIN DX88 TO TREAT ANGIOEDEMA IN PATIENTS WITH HEREDITARY ANGIOEDEMA 

Teresa Gonzalez-Quevedo, M.Cicardi. K Bork†, A. Williams§ 

222

COMPLEMENT REGULATION BY IMMOBILISED HEPARIN IS DEPENDENT ON SURFACE CONCENTRATION AND BLOOD VELOCITY

Jonas Andersson, Javier Sanchez, Kristina Nilsson Ekdahl, Graciela Elgue, Bo Nilsson, Rolf Larsson

238

A C1q PEPTIDE IS A POTENT C1 INHIBITOR

Haixiang Jiang, Huamei Zhang and Michael M. Frank

242

BACTERIAL EXPRESSION AND MEMBRANE-TARGETING OF RAT CD59 FOR USE IN ANTICOMPLEMENT THERAPY.

D.A. Fraser1, S. Gallagher2, C.L. Harris1, R.A.G. Smith2 and B.P. Morgan1.

295

AN ANTI-C3BI MAB ENHANCES COMPLEMENT ACTIVATION, C3BI DEPOSITION, AND KILLING OF CD20 POSITIVE CELLS BY RITUXIMAB (RTX).

Ronald P. Taylor*, Adam D. Kennedy*, Michael D. Solga*, Patricia L. Foley**, Margaret A. Lindorfer*, Theodore A. Schuman*, W. J. Amos Chi*, William M. Sutherland***:

179

PRE-CLINICAL EVALUATION OF THE VACCINIA VIRUS COMPLEMENT CONTROL PROTEIN (VCP) IN BLOCKING REJECTION FOLLOWING SURGICAL XENOTRANSPLANTATIONS.

G.J. Kotwal, J.A. Anderson, S. A Smith, R. Van Wijk and D. Kahn. 

47

CHEMICAL SYNTHESIS OF SMALL COMPLEMENT PROTEINS AND PROTEIN MODULES 

Lynn Spruce, Emelia Argyropoulos, Dimitrios Mastellos, Georgia Sfyroera, and John D. Lambris 

50

STRUCTURAL-ACTIVITY-BASED DESIGN OF COMPSTATIN USING ANALOGS NMR AND COMBINATORIAL APPROACHES. 

Dimitrios Morikis,1 Athena Soulika,2 John D. Lambris. 

Complement as diagnostic tool

310

A Novel COMPLEMENT-BASED Assay for SLE Diagnosis and Assessment of Disease Activity

JS Navratil, SM Manzi, MJ Ruffing, CC Liu, DE King, N Danchenko, PS Shaw, SE Nilson, and JM Ahearn

27

Comparison of anaphylatoxin C3a and C5a Effects on prostanoid mediated glucose release in the perfused rat liver

C. Wagner 1, S. Pestel 1, G. Schlaf 2, O. Götze 2, K. Jungermann 1, H.L. Schieferdecker 

204

EARLY DETECTION OF ORTHOPOXVIRUS INFECTION IN SERUM USING RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP) OF COMPLEMENT REGULATORY PROTEIN GENES 

Ariella M. Rosengard, Zhiping Nie, and Yu Liu 

231

CHARACTERIZATION OF ANTI-FACTOR H ANTIBODIES THAT ARE USED TO DETECT MARKERS OF BLADDER CANCER.

Zhu-Zhu Cheng*, Michael J. Corey#, Jens Hellwage+, Peter F. Zipfel+, Robert J. Kinders#, Seppo Meri*, and T. Sakari Jokiranta*.

9

NOVEL SPECIFIC ACTIVATION PRODUCTS OF THE CLASSICAL PATHWAY OF COMPLEMENT.

D. Wouters, H.D. Wiessenberg, M. Hart, A. Roos, M.R. Daha, C.E. Hack 

Recombinant Complement Proteins

51

MONOCLONAL ANTIBODIES AGAINST MOUSE C3-FRAGMENTS - A NEW REPERTOIRE OF REAGENTS TARGETING C3-MEDIATED FUNCTIONS 

A.Erdei*, G. László*, E. Oláh*, J. Prechl*, K. Papp*, E. Csomor, E. Argyropoulos+, S. Franchini+, D. Mastellos+, J.D. Lambris+ 

105

RECOMBINANT ANTI-COBRA VENOM FACTOR AND ANTI-HUMAN C3 ANTIBODIES SUITABLE FOR FUNCTIONAL ANALYSES  

Johanna Kölln, Edzard Spillner, Thorsten Mix, Mona Nagel, and Reinhard Bredehorst

121

A NOVEL ASSAY FOR FACTOR D ACTIVITY 

Etsuko Kitano, Emi Kobayashi, Yoshinobu Murakami*, Hiroo Iwata* & Hajime Kitamura, 

144

UTILITY OF COMPLEMENT RECEPTOR 1 (CR1) AND SOLUBLE COMPLEMENT RECEPTOR (sCR1) ASSAY IN THE DIAGNOSIS AND PROGNOSIS OF IMMUNE COMPLEX MEDIATED GLOMERULOPATHIES. 

L.M.Srivastava, K.R.Raju, B. Sivasankar, N.Das, S.C.Tiwari and A.K.Dinda. 

173

EXPRESSION AND CHARACTERIZATION OF WILD TYPE AND VARIANT HUMAN C1 INHIBITOR USING THE BACULOVIRUS VECTOR EXPRESSION SYSTEM 

. Shenghe Cai and Alvin E. Davis III.

184

RECOMBINANT HUMAN C1 INHIBITOR: PRODUCTION IN THE MILK OF TRANSGENIC RABBITS , 

Erik Smaal, Harrie van Veen, Jaco Koiter and Rene Verdonk

213

DEVELOPMENT OF A HIGH EFFICIENT METHOD TO PURIFY AND CRYSTALLIZE IMMUNOSUPPRESSIVE PROTEIN sCR1 FOR THE APPLICATION TO XANOGRAFT 

Hisamune Kato (Master program of Biosystem, Univ. Tsukuba) Noriyuki Ishii (National Inst. of Advanced Industrial Science and Technology, Tsukuba) Pi-Chao Wang (Inst, Appl. Biochem, Univ. Tsukuba) 

248

CHARACTERISATION OF THE MANNAN-BINDING LECTIN ASSOCIATED SERINE PROTEASE 3 AND ITS RECOMBINANT LARGE SCALE PRODUCTION IN EUKARYOTIC CELLS

Dahl, M.R.1, Thiel, S.2, Jensenius, J.C.2, Schwaeble, W.

11

EXPRESSION OF RECOMBINANT C1-INHIBITOR IN THE METHYLOTROPHIC YEAST PICHIA PASTORIS

Ineke G.A. Bos, Yani Karuntu, and C. Erik Hack

Complement and infections

70

THE YEAST AND HYPHAL FORMS OF CANDIDA ALBICANS BIND COMPLEMENT ALTERNATIVE PATHWAY REGULATORS FACTOR H AND FHL-1 

T. Meri1,2, A. Hartmann1, D. Lenk 1, R. Eck1, R. Würzner3, J. Hellwage2, S. Meri1 and P.F. Zipfel2 

316

Kaposi's sarcoma-associated herpesvirus (human herpesvirus-8) open reading frame 4 protein (Kaposica) is a functional homolog of complement control proteins

Jayati Mullick¹, John Bernet¹, Akhilesh Singh¹, John Lambris² and Arvind Sahu¹.

314

Localization of the Pneumococcal Surface Protein PspC Binding Site on the Human Complement Regulator Factor H TO SCR 13-15.

Thomas G. Duthy1, Rebecca J. Ormsby1, Eleni Giannakis1, A. David Ogunniyi2, Uwe H. Stroeher2, James C. Paton2 and David L. Gordon1

5

HUMAN C4B-BINDING PROTEIN (C4BP) BINDS WITH HIGH SPECIFICITY TO THE HYPERVARIABLE REGION OF MANY STREPTOCOCCAL M PROTEINS AND CONTRIBUTES TO PHAGOCYTOSIS RESISTANCE

Jenny Persson, Karin Berggård, Eva Morfeldt och Gunnar Lindahl. 

18

INHIBITION OF THE COMPLEMENT MEMBRANE ATTACK COMPLEX BY PARAMYOSIN OF SCHISTOSOMA MANSONI. 

Jiusheng Deng, Philip LoVerde2, Daniel Gold and Zvi Fishelson, 

19

C3 DEPOSITION INDUCED BY SALMONELLA SEROGROUP C-SPECIFIC OLIGOSACCHARIDE IN C2-DEFICIENT SERA: ACTIVATION OF THE ALTERNATIVE PATHWAY THROUGH THE MANNAN-BINDING LECTIN PATHWAY.  

Selander B*, Mårtensson U*, Weintraub A†, Holmström E*, Truedsson L*, Thiel S§, Jensenius JC§, Sjöholm AG*.

20

IgG ANTIBODY RESPONSES TO VACCINATION WITH CAPSULAR POLYSACCHARIDES IN C2 DEFICIENCY 

. Sjöholm AG*, Jönsson G§, Selander B*, Holmström E*, Sturfelt G#, Truedsson L*.

43

ACTIVATION OF CHICKEN COMPLEMENT BY BACTERIAL LIPOPOLYSACCHARIDES.  

T.L. Koppenheffer.

45

STUDIES ON THE INTERACTION OF VACCINIA COMPLEMENT CONTROL PROTEIN WITH C3 

Emelia Argyropoulos, Mohammad Shamim, Edward Bell, Stuart N. Isaacs, John D. Lambris 

81

COMPLEMENT RESISTANCE IN LYME DISEASE SPIROCHETES IS MEDIATED BY INDUCIBLE EXPRESSION OF MULTIPLE PLASMID-ENCODED OspE/ErpA PARALOGS 

Antti Alitalo1, Taru Meri1, Hilkka Lankinen2, Ilkka Seppälä1, Pekka Lahdenne1, P. Scott Hefty3, Darrin R. Akins3, and Seppo Meri1 

110

COMPLEMENT REGULATORY PROTEINS ATTENUATE THE FUNCTIONAL EFFECT OF ANTIBODY ELICITED BY A GONOCOCCAL VACCINE CANDIDATE 

. Jutamas Ngampasutadol, Sunita Gulati, Sanjay Ram and Peter A. Rice,

132

THE STREPTOCOCCAL BETA AND PNEUMOCOCCAL HIC PROTEINS UTILIZE HOMOLOGOUS MOTIFS FOR BINDING TO THE MIDDLE PART OF THE COMPLEMENT INHIBITOR FACTOR H 

Hanna Jarva*, Thomas Areschoug†, Jens Hellwage‡, Markus J. Lehtinen*, Peter F. Zipfel‡, Gunnar Lindahl† and Seppo Meri*

135

C4B-BINDING PROTEIN INTERACTIONS WITH NEISSERIA GONORRHOEAE PORIN IS INFLUENCED BY HEXOSE SUBSTITUTIONS ON THE HEPTOSE I CHAIN OF LIPOOLIGOSACCHARIDE. 

Ram S*,Boden R*, Stein DC¶, Cox AD‡, O’Reilly B*, Apicella MA§, Gulati S*, Blom AM** and Rice PA*. 

136

PHOSPHOETHANLOAMINE SUBSTITUTIONS ON NEISSERIAL LIPOOLIGOSACCHARIDE ARE THE PREFERRED ACCEPTORS FOR C4 

Ram S*, Cox AD¶, Vogel U**, Boden R*, Plested J§, Meri S‡, Getzlaff S**, Richards JC¶, Moxon ER§, and Rice PA*.

143

EFFECT OF C3D FUSION ON THE IMMUNOLOGICAL RESPONSE TO THE E2 PROTEIN OF BOVINE VIRAL DIARRHEA VIRUS (BVDV).  

Lingshu Wang, J. Oriol Sunyer and Leonard J. Bello.

159

COMPLEMENT PLAYS A CRUCIAL ROLE IN KILLING OF GROUP A STREPTOCOCCI IN THE PRESENCE OF OPSONISING ANTIBODIES 

Gustafsson M, Truedsson L, Sjöbring U 

163

CHARACTERIZATION, ENHANCEMENT AND BIOLOGICAL EFFECTS OF THE INTERACTION OF MANNOSE-BINDING LECTIN (MBL) WITH GP120 ON HIV.  

Gregory T. Spear, Melanie L. Hart and Mohammed Saifuddin.

175

THE INTERACTION OF C1 INHIBITOR WITH GRAM NEGATIVE ENDOTOXIN.  

Dongxu Liu,Xiagnag Gu, Jennifer Scafidi, and Alvin E. Davis III.

193

COMPLEMENT IN MURINE PSEUDOMONAS PNEUMONIA: IN VITRO INTERACTIONS AND IN VIVO EFFECTS OF COMPLEMENT DEPLETION. 

John G. Younger, Sunita Shankar-Sinha, Adam S. Brinkman, Marc Mickiewicz, Ted J. Standiford, and Peter A. Ward.

203

Mapping of complement binding sites within the Smallpox Inhibitor of Complement Enzymes (SPICE) and Vaccinia Virus complement control Protein (VCP)  

ARIELLA M. ROSENGARD, YU LIU, JEREMY REID, DILEK AVCI, ROBERT JIMENEZ

215

48 MICROORGANISM-INDUCED MANNOSE-BINDING LECTIN (MBL) ACTIVATION  

S. Kuipers1, P.C. Aerts1,2, M. Harmsen1, and H. van Dijk1

217

THE ADHERENCE OF HIV TO ERYTHROCYTES IS COMPLEMENT DEPENDENT IN AN IN VITRO MODEL.  

Eliska Horakova1, Olivier Gasser1, Salima Sadallah1, Thomas Klimkait2, Jürg A. Schifferli1

233

ROLE OF COMPLEMENT IN A PHASE 1 CLINICAL TRIAL WITH MONOCLONAL ANTIBODIES DIRECTED AGAINST HIV-1

Christoph G. Ammann1, Susanne G. Döpper1 , Gabriele Stiegler2, Brigitte Mullauer1, Brigitta Vcelar2, Hermann Katinger2, Christine Ambruster3, Norbert Vetter3, Manfred P. Dierich1and Heribert Stoiber1

234

FUNCTIONAL CHARACTERISATION OF THE HUMAN HERPESVIRUS 8 COMPLEMENT REGULATING PROTEIN HOMOLOGUE

O. Brad Spiller1, B. Paul Morgan1, Mairi Robinson2, Liz O’Donnell2, Charles Cunningham2, Andrew Davison2, David J. Blackbourn2

235

PRESENCE OF COMPLEMENT COMPONENT C3 AND THE ALTERNATIVE PATHWAY ARE CRITICAL FOR A PROTECTIVE IMMUNE RESPONSE AGAINST PSEUDOMONAS AERUGINOSA IN A MURINE MODEL OF PNEUMONIA INFECTION

Stacey L. Mueller-Ortiz, Scott M. Drouin, and Rick A. Wetsel.

236

COMPLEMENT ACTIVATION INFLUENCES STAPHYLOCOCCAL ADHERENCE TO ENDOTHELIAL CELLS

K.M. Cunnion and M.M. Frank

243

THE PRESENCE OF COMPLEMENT COMPONENT C3 IS IMPORTANT IN GENERATING A LOCALIZED INFLAMMATORY RESPONSE TO CUTANEOUS STAPHYLOCOCCUS AUREUS INFECTION.

David L. Haviland1, Eric Brown2, Magnus Hook2, and Rick A. Wetsel1.

249

REGULATION OF THE MANNAN-BINDING LECTIN PATHWAY OF COMPLEMENT ON PATHOGENIC NEISSERIA BY C1-INHIBITOR AND a-2 MACROGLOBULIN

Sunita Gulati*ý, Kedarnath SastryÝ, Jens C. Jenseniusý, Peter A. Rice* and Sanjay Ram*.

256

COMPLEMENT-MEDIATED INTERNALISATION OF HUMAN URINARY PATHOGENS IS STRAIN DEPENDENT AND RELATED TO TYPE 1 FIMBRIATION

Sheerin NS, Li K, Springall T, Hong Y, Sacks SH

266

DETERMINATION OF COMPLEMENT LEVELS IN HIV PATIENTS

Ischenko A.1, Prilutsky A.2, Trofimov A.1, Zhakhov A.1, Maylian E.2, Lysenko K.2, Rodin S.1, Seliverstova V.

268

STRUCTURAL ANALYSIS OF THE INTERACTION BETWEEN M5 PROTEIN AND FACTOR H DOMAIN 7 AND IDENTIFICATION OF A DISTICT M PROTEIN BINDING SITE ON DOMAINS 8-15.

T. S. Jokiranta1, E. Giannakis2, R. Ormsby2, G. Lindahl3, and D. L. Gordon2.

271

COMPLEMENT RESISTANCE OF BORRELIA BURGDORFERI: MOLECULAR CLONING OF A FACTOR H BINDING PROTEIN AND LOCALIZATION OF THE BINDING DOMAIN

P. Kraiczy1, J. Hellwage2, C. Skerka2, M. Kirschfink3, V. Brade1, P.F. Zipfel2 and R. Wallich3

272

L-FICOLIN BINDING TO DIFFERENT MONOSACCHARIDES AND MICROORGANISMS.

Anders Krarup1, Steffen Thiel1, Misao Matsushita2, Uffe B. Skov Sørensen1 and Jens Chr. Jensenius1.1)

276

BORRELIA RECURRENTIS EVADES COMPLEMENT ALTERNATIVE PATHWAY ACTIVATION BY BINDING FACTOR H AND FHL-1.

T. Meri1, S. J. Cutler2, J. Hellwage3, P. F. Zipfel3, S. Meri1, T. S. Jokiranta1

291

IMPAIRED ANTIBODY RESPONSE TO GROUP B STREPTOCOCCAL TYPE III CAPSULAR POLYSACCHARIDE IN COMPLEMENT C3- AND COMPLEMENT RECEPTOR 2-DEFICIENT MICE.

Olga Pozdnyakova,*† Hilde-Kari Guttormsen,*‡ Farah N. Lalani,‡ Dennis L. Kasper§2 and Michael C. Carroll,†

309

THE MECHANISM OF COMPLEMENT RESISTANCE MEDIATED BY THE OUTER MEMBRANE PROTEIN  DSRA

Abdullah, M. T., Olsen, B., and Elkins, C

303

IMPORTANCE OF THE TERMINAL COMPLEMENT COMPONENTS FOR IMMUNE DEFENCE AGAINST CANDIDA.

T. Triebel, B. Grillhösl, L. Kacani, C.P. Lell, A. Fuchs, C. Speth, C. Lass-Flörl, J. Steinmann, M.P. Dierich, R. Würzner.

THURSDAY SEPTEMBER 26

Complement and Apoptosis

155

THE EFFECT OF TERMINAL COMPLEMENT COMPLEX ON APOPTOSIS GENE EXPRESSION IN EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS 

H. Rus*, S. Weerth‡, L. Soane*, F. Niculescu*, V. Rus*, C. Raine‡ and M.L. Shin*. 

10

COMPLEMENT ACTIVATION BY APOPTOTIC CELLS IN HUMAN PLASMA.

C.L.F. Ciurana, B. Zwart, I. Rensink, R Manoe, C.E. Hack, L.A. Aarden

44

SIGNAL TRANSDUCTION THROUGH ACTIVATED C3d/EBV RECEPTOR (gp140, CR2, CD21) ON HUMAN B LYMPHOCYTE SURFACE: pp60src IS THE TYROSINE KINASE WHICH PHOSPHORYLATES THE 95 kDa NUCLEOLIN UPSTREAM PI 3-KINASE ACTIVATION, IN A CD19 INDEPENDENT PATHWAY.. 

Monique BAREL and Raymond FRADE

116

FIBRONECTIN FRAGMENT ASSOCIATING C4b-BINDING PROTEIN 

Yasuo Hitsumoto and Mariko Okada*. 

280

MANNOSE-BINDING LECTIN (MBL) TAGS APOPTOTIC CELLS AND CELL BLEBS AS WELL AS NECROTIC CELLS

A.J. Nauta1, N. Raaschou-Jensen2, P. Garred2, M.R. Daha1, H.O. Madsen2, L. P. Ryder2, C. Koch3, M. Borrias1, and A. Roos1

281

PENTRAXIN 3 CAN ACTIVATE THE CLASSICAL COMPLEMENT PATHWAY; A POTENTIAL SYNGERGISTIC ROLE FOR PTX3 AND COMPLEMENT IN THE CLEARANCE OF APOPTOTIC CELLS

Alma J. Nauta1, Barbara Bottazzi2, Alberto Mantovani2, 3, Wilhelm J. Schwaeble4, Alexandre R. Gingras4,  Sotiria Tzima4, Fernando Vivanco5, Jesús Egido6, Odette Tijsma1, Erik C. Hack7, Mohamed R. Daha1, and Anja Roos1

289

MURINE CD93 (C1qRP) DEFICIENCY CAUSES A DEFECT IN APOPTOTIC CELL CLEARANCE IN VIVO.

Norsworthy PJ., Fossati-Jimack L., Cortes- Hernandez J., Taylor P., Bygrave A., Walport MJ., and Botto M.

Immune Regulation

308

The role of a newly-identified heterologous crosstalk between G-protein coupled seven transmembrane span receptors - chemokine receptor CXCR4 and complement C3aR in human hemato-lymphopoiesis and innate immunity

Mariusz Z. Ratajczak, Ryan Reca, Marcin Majka, Jacek Kijowski, Dimitrios Mastellos, Silvia Franchini, John D. Lambris

59

TARGETING ANTIGEN TO CR1/2 BY SINGLE-CHAIN ANTIBODY FRAGMENTS RESULTS IN LOW AFFINITY IgM RESPONSE WITHOUT THE INDUCTION OF MEMORY 

József Prechl&, Eszter Molnár, Andrea Isaák, Krisztián Papp, Anna Erdei& 

89

DYSFUNCTIONAL PERIPHERAL B CELLS DEVELOP IN TRANSGENIC MICE THAT PREMATURELY EXPRESS HUMAN COMPLEMENT RECEPTOR TYPE 2 (CR2/CD21) DURING EARLY B CELL DEVELOPMENT.  

Liudmila Kulik1, Kevin J. Marchbank 2, B. Paul Morgan2 and V. Michael Holers1

95

COMPLEMENT RECEPTOR TYPE 2 (CR2) ACTS AS A CO-RECEPTOR ON B-1 CELLS AND INCREASES B CELL RECEPTOR MEDIATED CALCIUM MOBILIZATION IN A SIMILAR FASHION AS CONVENTIONAL B CELLS. 

Taras Lyubchenko1, Adam Zwiefach2, and V. Michael Holers1 

98

C4 DEFICIENCY MODULATES NEGATIVE SELECTION OF AUTOREACTIVE B CELLS. 

Elahna Paulab and Michael C. Carrollbc 

127

AUGMENTATION OF THE ALLORESPONSE BY COMPLEMENT ACTIVATION ON NON-ANTIGEN PRESENTING CELLS -RENAL TUBULAR EPITHELIAL CELLS W.  

Zhou, K Li, C.A. Farrar, S. Jurcevic, S.H. Sacks,

128

CO-OPERATION BETWEEN CR1/CD35 AND CR2/CD21 IN THE BINDING OF iC3 TO HUMAN B LYMPHOCYTES., 

R. Graham Q. Leslie1, Claus H. Nielsen2 and Wolfgang M. Prodinger3

151

SERUM COMPLEMENT ACTIVATION IS ASSOCIATED WITH DIMINISHED HUMAN T LYMPHOCYTE RESPONSES IN VITRO.  

R.D.R. Camp, J.M. Hales, 1P.P. Freestone and D.S. Cundell.

156

ROLE OF CD19 CYTOPLASMIC TYROSINES IN B LYMPHOCYTE FUNCTION IN VIVO.  

Robert H. Carter, Robert Rickert, and Yue Wang,

162

EXPRESSION OF HUMAN COMPLEMENT RECEPTOR TYPE 2 (CR2/CD21) IN MICE DURING EARLY B CELL DEVELOPMENT RESULTS IN A REDUCTION IN MATURE B CELLS AND HYPOGAMMAGLOBULINEMIA. 

Kevin J. Marchbank1, Liudmila Kulik2, Matthew G. Gipson2, B. Paul Morgan1 and V. Michael Holers2 

186

CO-STIMULATION OF T CELLS THROUGH COMPLEMENT REGULATORY PROTEINS IN KIDNEY TRANSPLANT REJECTION 

H.P.Patel1; J.R.Pratt1; S.H.Sacks1 

187

CD46/CD3-ACTIVATION OF HUMAN CD4+ T CELLS INDUCES A T-REGULATORY 1 (TR1) PHENOTYPE.  

C. Kemper*, A. C. Chan†, J. M. Green‡, K. A. Brett*, K. M. Murphy||‡ and J. P. Atkinson*

200

CR2 DEFICIENCY RESULTS IN ALTERED RECEPTOR REVISION IN PERIPHERAL B CELLS. 

Susan A. Boackle1, Kristin K. Culhane1, Jared M. Brown1, David Nemazee2, and V. Michael Holers1. 

210

REGULATION OF NEUTROPHIL RECRUITMENT DURING IMMUNE COMPLEX PERITONITIS THROUGH C-X-C CHEMOKINES KC AND MIP-2 FROM MAST CELLS, COMPLEMENT AND IL-10.  

Jeanne Godau*, Tanja Heller*, Melanie Mueller*, Heiko Hawlisch*, Andreas Klos*, Nico van Rooijen†, and Jörg Köhl*.

214

ANTIGEN LOCALIZATION ON MARGINAL ZONE MACROPHAGES (MZM) RESTORES HUMORAL IMMUNE RESPONSE AND IGG CLASS SWITCH IN COMPLEMENT C4 DEFICIENT MICE 

B. Zachrau1, K. Kropf1, D. Finke1, M.C. Carroll2, M. Ma2, H. Kirchner1, and S. Goerg1 

224

A ROLE FOR THE Cr2 GENE IN MODIFYING AUTOANTIBODY PRODUCTION IN SLE

Xiaobo Wu, Christine Deppong, Gregory Dolecki, Dailing Mao, Laurence Morel, and Hector Molina

225

IMPORTANCE OF CD21/CD35 AND FC_RIIB IN B LYMPHOCYTE MEMORY.

RA Barrington1, OO Pozdnyakova1, M Zafari2, C Benjamin2 and MC Carroll1.

251

RECONSTITUTION OF C4-DEFICIENT MICE WITH WILD TYPE BONE MARROW RESTORES HUMORAL IMMUNITY.

Mihaela Gadjeva 1, Admar Verschoor 1 and Michael Carroll 1,2,

254

ADEQUATE ACQUISITION OF CLONAL ANERGY BY CR2-DEFICIENT SELF-REACTIVE B CELLS IN A TRANSGENIC MODEL OF IMMUNOLOGICAL

Xiaobo Wu and Hector Molina.

269

INEFFICIENT ACTIVATION OF T LYMPHOCYTES BY GPI-ANCHOR-NEGATIVE CELLS: IMPLICATIONS FOR PAROXYSMAL NOCTURNAL HEMOGLOBINURIA

Taroh Kinoshita, Yoshiko Murakami, Yusuke Maeda, Jun-ichi Nishimura and Norimitsu Inoue.

302

DISCRETE FUNCTIONALITY FOR COMPLEMENT C3 FROM DISTINCT SOURCES.

Admar Verschoor*‡, Mark A. Brockman†, David M. Knipe† and Michael C. Carroll‡§

305

COMPLEMENT RECEPTORS CD21/CD35 ARE IMPORTANT IN SHAPING THE REPERTOIRE OF NATURAL IGM PRODUCING B-1 CELLS.

Ming Zhang, Russell Reid, William Austin, Jr., Min Xu, Sean Woodlock, Les Kobzik, Herbert B. Hechtman, Francis D. Moore, Jr., and Michael C. Carroll

Animal Models

311

TARGETED DELETION OF MOUSE Cd59b REPRODUCES THE HEMATOLOGICAL FEATURES OF HUMAN PAROXYSMAL NOCTURNAL HEMOGLOBINURIA (PNH).

X. Qin, N. Krumrei, L. Grubissich, G. Perez, M. Dobarro, S. Sledge, J. Halperin.

160

C5A AS A PROBE FOR UNDERSTANDING THE COMPLICATIONS IN EXPERIMENTAL SEPSIS.F.S. 

Zetoune, P.A. Ward, R.F. Guo, T.A. Neff, V. Sarma, E.M. Younkin, and N.C. Riedemann. 

4

PATHOLOGIC SIGNIFICANCE OF AUTOANTIBODIES AGAINST C1Q IN THE DEVELOPMENT OF RENAL DISEASE.

Leendert A. Trouw, Jacques M.G.J. Duijs, Mohamed R. Daha 

23

UNCONTROLLED C3 ACTIVATION CAUSES MEMBRANO-PROLIFERATIVE GLOMERULONEPHRITIS IN FACTOR H-DEFICIENT MICE  

Botto M, Pickering, MC, 1Cook HT, Warren J, Bygrave AE, 1Moss J, Walport MJ.

32

RECONSTITUTION OF C1q-DEFICIENCY WITH BONE MARROW DERIVED CELLS AMELIORATES THE AUTOMMUNITY ASSOCIATED WITH C1q-DEFICIENCY  

M Botto, J Cortes-Hernandez, L Fossati-Jimack, 1S Izui, 2F Petry, 2M Loos, MJ Walport.

39

THE MANNOSE-BINDING LECTIN – SEVERE ATHEROSCLEROSIS PARADOX SOLVED? 

Saskia Kuipers, Piet Aerts, Bertie Dekker, Theo Harmsen, Arno van Oostrom, Caroline Verseyden, Manuel Castro Cabezas & Hans van Dijk*, 

46

PROTEOMIC ANALYSIS OF MOUSE C3 DEFICIENCY AND LIVER PARTIAL HEPATECTOMY  

C.W. Strey, D. Spellman, M. Markiewski and J.D. Lambris

48

THE ROLE OF COMPLEMENT IN LIVER REGENERATION  

C. W. Strey1,*, M. Markiewski1,*, D. Mastellos1,*, R. Tudoran1, L. Greenbaum2 and John D. Lambris1

49

THE ROLE OF COMPLEMENT IN NEWT LENS AND LIMB REGENERATION 

Kimura Y1., Lambris J.D1., Tsonis, P.A2, Del Rio-Tsonis K3 

53

LACK OF A FUNCTIONAL ALTERNATIVE COMPLEMENT PATHWAY PROTECTS MICE FROM RENAL ISCHEMIA/REPERFUSION INJURY. 

Joshua M. Thurman,1 Danica Ljubanovic,2 V. Michael Holers. 

58

DECAY-ACCELERATING FACTOR (CD55) IS ABUNDANTLY EXPRESSED BY REACTIVE NEURONS IN RESPONSE TO CHRONIC BUT NOT ACUTE INFLAMMATORY INSULTS ASSOCIATED WITH STRONG COMPLEMENT ACTIVATION. 

Johan van Beek1, Marjan van Meurs2, Bert A. ‘t Hart3, Herbert P.M. Brok3, Jim W. Neal4, B. Paul Morgan1, Jon D. Laman2 and Philippe Gasque4 

60

CD93 (alias C1qRp), a lectin-like receptor implicated in innate immune defense and adhesion, is abundantly expressed by neurons following CNS stroke inflammation. 

Yann D. Dean1, Johan van Beek1, Helene Nallet2, Jim W. Neal3 and Philippe Gasque1 

69

SOLUBLE DAF ABROGATES CVB3-INDUCED MYOCARDITIS IN MICE. 

O. Brad Spiller1, Bobby Yanagawa2, Bruce M. McManus2 

73

ALLOSTIMULATION THROUGH LOCALLY SYNTHESIZED COMPLEMENT C3 IN KIDNEY TRANSPLANTS 

. S.A. Basheer, J.R. Pratt & S.H. Sacks.

90

ANOXIA/REOXYGENATION PRECIPITATES COMPLEMENT DEPOSITION ON RAT CARDIAC MYOCYTES BY A LECTIN-DEPENDENT PATHWAY  

Charles Blais, Jr., Todd Bourcier, Gregory L. Stahl*

91

DELETION OF DECAY-ACCELERATING FACTOR (DAF, CD55) EXACERBATES AUTOIMMUNE DISEASE DEVELOPMENT IN MRL/LPR MICE  

T.Miwa, M.A. Maldonado, L. Zhou, H.Y. Luo, V.P. Werth, M.P. Madaio, R.A.

104

COMPLEMENT IS NOT ESSENTIAL FOR THE DEVELOPMENT OF NEURITIC (SENILE) PLAQUES IN AN ANIMAL MODEL OF ALZHEIMER’S DISEASE.  

N.R. Cooper1, B.M. Bradt1, K. Hsiao2, M.C. Carroll3, J.X. Yu1.

112

ABSENCE OF THE COMPLEMENT ANAPHYLATOXIN C3A RECEPTOR AND THE ALTERNATIVE PATHWAY COMPONENT FACTOR B SUPPRESSES TH2 EFFECTOR FUNCTIONS IN A MURINE MODEL OF PULMONARY ALLERGY.  

Scott M. Drouin1, David B. Corry2, Jens Kildsgaard1, Travis J. Hollmann1, and Rick A. Wetsel1.

133

THE ADMINISTRATION OF COMPLEMENT COMPONENT C9 AUGMENTS HYPOXIC-ISCHEMIC CEREBRAL INFARCT VOLUME IN NEONATAL RATS  

Herbert A. Lassiter, Mitchell D. Imm, Pamela W. Feldhoff, and Richard C. Feldhoff,

138

MECHANISMS FOR C1-INHIBITOR NEUROPROTECTION IN AN ANIMAL MODEL OF TRANSIENT FOCAL BRAIN ISCHEMIA.  

M.G. De Simoni, C. Storini, E. Rossi*, A. Agostoni*, R. Veerhuis**, and L. Bergamaschini*.

154

COMPLEMENT C5 ACTIVATION MEDIATES ANTIPHOSPHOLIPID ANTIBODY-INDUCED PREGNANCY LOSS.  

Guillermina Girardi, Jessica Berman, Lynn Spruce*, John D Lambris*, Michael Holers# and Jane E. Salmon.

170

CONTRIBUTION OF ANAPHYLATOXIN C5A TO ALLERGIC LATE AIRWAY RESPONSES AFTER REPEATED ANTIGEN EXPOSURE 

Masayoshi Abe,* Kazuhiko Shibata,† Hiroyasu Akatsu,§ Naomi Shimizu,† Takeshi Katsuragi,* and Hidechika Okada§

183

HALF LIFE AND ACTIVITY OF CARBOXYPEPTIDASE R OF EXPERIMENTAL ANIMALS 

Hidefumi Komura1, 2, Yasuyo Shimomura2, Hirotada Katsuya1, Noriko Okada2, and Hidechika Okada2 

194

ASTROCYTE-TARGETED EXPRESSION OF THE SOLUBLE MURINE COMPLEMENT INHIBITOR sCRRY MEDIATES NEUROPROTECTION AFTER EXPERIMENTAL TRAUMATIC BRAIN INJURY IN TRANSGENIC MICE. 

P.F. Stahel1, M. Rancan2, M.C. Morganti-Kossmann2, S. Saft1, W. Ertel1, S.R. Barnum3. 

201

SPECIFIC PHENOTYPES INDUCED BY DEFICIENCY OF COMPLEMENT RECEPTORS TYPE 1 (CR1/CD35) AND TYPE 2 (CR2/CD21) IN THE MRL/lpr MURINE MODEL OF SLE. 

Susan A. Boackle1, Kristin K. Culhane1, Jared M. Brown1, Mark Haas2, Lihua Bao3, Richard J. Quigg3 and V. Michael Holers1. 

207

A CENTRAL ROLE FOR FC_RIIB AND C5A IN NEUTROPHIL RECRUITMENT DURING IMMUNE COMPLEX PERITONITIS.  

Tanja Heller*, Jeanne Godau*, Melanie Mueller*, Sjef Verbeek†, Heiko Hawlisch*, Andreas Klos*, and Jörg Köhl*.

211

C5A TRIGGERS THE CONTACT HYPERSENSITIVITY IN MICE AND IS A POTENTIAL THERAPEUTIC TARGET.  

H.Hawlisch*, T. Heller*, T. Tschernig†, A. Klos* and J. Köhl*.

218

CRIT-H17 PEPTIDE INHIBITS INFLAMMATION IN THE REVERSED PASSIVE ARTHUS REACTION 

. Marta Armanini1, Brigitte Schneider2, Jürg A.Schifferli2 & Jameel M. Inal2

223

COMPLEMENT ANAPHYLATOXINS C3A AND C5A MODULATE INTRACELLULAR Ca2+ SIGNALLING AND EXCITABILITY OF CA1 PYRAMIDAL NEURONS: SIMULTANEOUS Ca2+ IMAGING AND WHOLE-CELL RECORDING IN RAT HIPPOCAMPAL ORGANOTYPIC SLICES.

Antonio Campos-Torres(1), Lucas Pozzo-Miller(2), Eve McCutchen(2) and Scott Barnum(1).

227

REDUCED C3 SERUM LEVELS IN ADULT CRRY-DEFICIENT MICE DUE TO AN INCREASED C3 TICKOVER AND SPONTANEOUS ACTIVATION OF THE ALTERNATIVE PATHWAY

Dailing Mao, Xiaobo Wu, and Hector Molina

240

A DISPENSABLE ROLE FOR NEUTROPHILS, C5, AND THE CLASSICAL PATHWAY OF COMPLEMENT ACTIVATION IN THE ABNORMAL FETOMATERNAL TOLERANCE FOUND IN CRRY-DEFICIENT MICE  

Dailing Mao, Xiaobo Wu, and Hector Molina

246

EFFECT OF COMPLEMENT FACTOR D DEFICIENCY ON RENAL DISEASE IN MRL/LPR MICE.

Margaret Elliott, Philip Ruiz, Yuanyuan Ma, Gary Gilkeson.

257

IN VIVO DYNAMICS OF IMMUNE COMPLEX PROCESSING THROUGH COMPLEMENT AND Fc_ƒnRECEPTORS

Xiaobo Wu, Dailing Mao and Hector Molina

260

DECAY ACCELERATING FACTOR DEFICIENCY INCREASES SUSCEPTIBILITY TO DEXTRAN SULFATE SODIUM-INDUCED COLITIS IN MICE.

Feng Lin,1 David Spencer,2 Alan D. Levine, 2 and M. Edward Medof1,

261

ENHANCED SUSCEPTIBILITY TO MYASTHENIA GRAVIS IN THE ABSENCE OF DECAYACCELERATING FACTOR PROTECTION.

Feng Lin,1 Henry J. Kaminski,2 Bianca M. Conti-Fine,3 Wei Wang,3 Chelliah Richmonds,2 and M. Edward Medof 1

274

EXACERBATION OF EAE IN MICE OVER-EXPRESSING THE COMPLEMENT ANAPHYLATOXIN C3a IN THE CNS

Laura Boos1, Robert Ames2, Iain Campbell4, and Scott R. Barnum1

290

INHIBITION OF C1q-_-AMYLOID BINDING PROTECTS HIPPOCAMPAL CELLS AGAINST COMPLEMENT-MEDIATED TOXICITY

Sárvári1, M., Gál2, P., Vágó1, I., Wéber1, Cs., Nagy1, J., Kósa1, J.P., Závodszky2, P. and Pázmány1, T.

293

MUCOSAL IMMUNIZATION WITH MOG 35-55 IN MICE THAT OVEREXPRESS THE COMPLEMENT REGULATORYMOLECULE SCRRY IN THE CNS PROVIDES ENHANCED PROTECTION AGAINST EAE

Rachael Reiman and Scott R. Barnum,

294

CD59-DEFICIENCY EXACERBATES CLINICAL DISEASE AND CENTRAL NERVOUS SYSTEM INFLAMMATION IN MURINE EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS.

Richard J. Mead, James W. Neal and B. Paul Morgan.

 Human Pathology

124

KEY ROLE OF THE C5a RECEPTOR IN E. COLI-INDUCED OXIDATIVE BURST AND PHAGOCYTOSIS STUDIED IN A NOVEL HUMAN WHOLE BLOOD MODEL OF INFLAMMATION 

O.L. Brekke1, H. Fure2, D. Christiansen2, K.T. Lappegård3, M. Fung4, J.D. Lambris5 and T.E. Mollnes2,6 

318

Human IgM monoclonal antibodies against HIV-infected cells and activated T lymphocytes

Noriko Okada, Shyun Yin, Suzuka Asai, Natsuki Dohi and Hidechika Okada

153

THE CHANGE OF COMPLEMENT SYSTEM PREDICTS THE OUTCOME OF PATIENTS WITH SEVERE THERMAL INJURY.  

Hee Jung Kang, Jong Hyun Kim*, Yung Kyoung Lee, Kyu Man Lee

12

IMPAIRED FIXATION OF COMPLEMENT C4 TO PENTRAXINS IN A RA PATIENT,

A. Familian1, G.J. van Mierlo1, A.E. Voskuyl3, J.A. Heijst2, B.A.C. Dijkmans3, C.E. Hack1

15

Factor H Gene Mutations In Hemolytic Uremic Syndrome: Reduced Protein Interactions Reveal A Role For Microvascular Damage And Thrombus Formation  

T. Manuelian1), J. Hellwage1), Seppo Meri2), Jessica Caprioli3), Marina Noris3), Hartmut P.H. Neumann4), Giuseppe Remuzzi3) and Peter F. Zipfel1),

34

GENETIC POLYMORPHISMS AND INCREASED SUSCEPTIBILITY FOR SYSTEMIC LUPUS ERYTHEMATOSUS: ANALYSIS OF HLA-DR3, MBL, IL-1RA AND FcGAMMARIIA GENES. 

A Jönsen1, AA Bengtsson1, L Truedsson2, G Sturfelt1 

40

IDENTIFICATION OF RCA HAPLOTYPES ASSOCIATED TO HIGH AND LOW LEVELS OF C4BP ISOFORMS CONTAINING _ CHAIN. 

Esparza-Gordillo, Jorge and Rodríguez de Córdoba, Santiago. 

62

MOLECULAR STUDIES IN TWO SPANISH FAMILIES WITH FACTOR I DEFICIENCY. 

Ponce I M, González-Rubio C, Delgado E, Fontán G, López-Trascasa M. 

66

LOW SERUM LEVELS OF MANNOSE-BINDING LECTIN MAY BE PROTECTIVE AGAINST CLINICAL TUBERCULOSIS. 

 C. Søborg 1, Å.B. Andersen 2, H.O. Madsen 1, T. Lillebæk 3, A. Kok-Jensen 4 & P.Garred1 

72

INCREASED EXPRESSION OF DECAY-ACCELERATING FACTOR BUT NOT CD59 ON PERIPHERAL BLOOD LEUCOCYTES FROM PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS AND RHEUMATOID ARTHRITIS 

A L Hepburn, K A Davies and J C Mason1 

85

CONGENITAL ADRENAL HYPERPLASIA DUE TO CLASSIC 21-HYDROXYLASE (CYP21) DEFICIENCY: POSSIBLE ASSOCIATION WITH C4 DEFICIENCY  

Guerra Jr. G.1; Nascimento SRD. 2; Araujo M. 2 ; Condino Neto A.1; Lemos-Marini, SHV1; Arruk V3; Kirschfink M4; Grumach AS 3 & De Mello MP2 .

97

PSEUDOALLERGY TO LIPOSOMAL AND MICELLAR FORMULATIONS OF INTRAVENOUS DRUGS: EXPERIMENTAL AND CLINICAL EVIDENCE FOR A NOVEL CATEGORY OF COMPLEMENT-MEDIATED ADVERSE REACTIONS 

J. Szebeni1, L. Baranyi1, S. Savay1, J. Milosevits1, A.A. Chanan-Khan2, F.M. Muggia2 , R.R.Cohen3, Y. Barenholz3 , R. Bunger4 and C.R. Alving1 

100

COMPLEMENT C3 DEFICIENCY AND EVIDENCE OF NONSENSE-CODON MEDIATED DECAY 

1Reis, E.S.; 2Nudelman, V. and 1Isaac, L. 

103

HOMOZYGOUS FACTOR H DEFICIENCY 

1Grumach, A.S., 1Vasconcelos, D.M., 1Arruk, V., 1Chagas, K.D.N., 2Brandão, A., 2Simonsen, V., 3Florido, M.P.C., 1Duarte, A.J.S. and 3Isaac, L. 

106

A ROLE OF GLYCATED HUMAN CD59 AND THE COMPLEMENT SYSTEM IN THE PATHOGENESIS OF CHRONIC VASCULAR COMPLICATIONS OF DIABETES.  

X.Qin, N. Kumrei, A. Goldfine, L. Grubuissich, and J. Halperin.

114

CONGENITAL ADRENAL HYPERPLASIA DUE TO CLASSIC 3_-HYDROXYSTEROID DEHYDROGENASE DEFICIENCY ASSOCIATED WITH LOW SERUM C4 LEVELS AND MOLECULAR ALTERATION ON C4 GENES.  

Guerra Jr G1; Nascimento SRD2; Araujo M2, Condino Neto, A.1; Lemos-Marini, S.H.V.1; Arruk V3; Kirschfink M4; Grumach, AS 3 & De Mello, MP2

118

A QUANTITATIVE GENETIC TRAIT FOR HUMAN LUPUS: LOW AND HIGH GENE DOSAGES AND PROTEIN EXPRESSION OF COMPLEMENT C4 IN SLE PATIENTS.  

Y Yang,1,2 EK Chung,1,2 BP Tsao,3 B Zhou,1 GC Higgins,1 RM Rennebohm,1 CA Blanchong,1 N Longman-Jacobsen,1 DJ Birmingham,2 BH Rovin,2 LA Hebert2 and CY Yu1,2.

119

COMPLETE OR PARTIAL DEFICIENCY OF HUMAN COMPLEMENT C4B IS A SIGNIFICANT RISK FACTOR OF PEDIATRIC CHRONIC IMMUNE THROMBOCYTOPENIA PURPURA (ITP).  

Carol A Blanchong,1 Frederick B Ruymann,1 Kathryn J Klopfenstein,1 Robert M Rennebohm,1 Yan Yang,1,2 Erwin K Chung,1,2 Bi Zhou,1 and C. Yung Yu 1,2.

122

E-CR1 EXPRESSION AND POLYMORPHISM IN INDIAN SUBJECTS 

Monika Katyal*, S. C. Tiwari., Ashok Kumar., A. K. Dinda. & Nibhriti Das* 

139

MUTATION SCREENING IN PATIENTS WITH HEREDITARY ANGIOEDEMA.  

E Pappalardo, MA Terlizzi, A Folcioni, LC Zingale and M Cicardi.

141

C1 INHIBITOR CONCENTRATE: EFFICACY AND ADVERSE REACTIONS.  

L.C. Zingale, E. Pappalardo, A. Zanichelli, A. Agostoni, M. Cicardi.

171

NEW MUTATIONS IN THE C1 INHIBITOR GENE IN PATIENTS WITH HEREDITARY ANGIOEDEMA. 

Blanch, A, Roche O, Fontán G, López-Trascasa M. 

177

MODULATION OF CR1 EXPRESSION IN GLOMERULONEPHRITIS 

Nibhriti Das*, B. Sivasankar*, S C Tiwari**, A K Dinda***, L.M. Srivastava****. 

208

LOCALIZATION OF THE HOST RECOGNITION FUNCTIONS OF COMPLEMENT FACTOR H AT THE CTERMINAL: IMPLICATIONS FOR HEMOLYTIC UREMIC SYNDROME  

Michael K. Pangburn,

219

PREVALENCE OF ANTIC1q AUTOAb

S. Sadallah, M. Trendelenburg, S. Regenass, C. Vedeler and JA. Schifferli

228

C4A/C4B POLYMORPHISMS IN KAWASAKI DISEASE.

Barry L Myones1, Stanford T Shulman2, Joann M Moulds3.

230

GLYCATION OF CD59 IMPAIRS COMPLEMENT REGULATION ON ERYTHROCYTES FROM DIABETIC SUBJECTS. 

Catherine S. Davies1*, Claire L. Harris2 & B. Paul Morgan2

245

GLOMERULAR DEPOSITION AND URINARY EXCRETION OF COMPLEMENT FACTOR H IN IDIOPATHIC MEMBRANOUS NEPHROPATHY

Morito Endo, Yoshinobu Fuke, Mariko Tamano, Mutsuko Hidaka, Isao Ohsawa, Takayuki Fujita and Hiroyuki Ohi