- I ORAL SESSION - Mechanism of Activation

 - II ORAL SESSION - Complement Regulators

 - POSTER SESSION

 - I ORAL SESSION - Genetics and Structure

 - II ORAL SESSION - Immune Modulation

 - I ORAL SESSION - Pathology 1

 - II ORAL SESSION - Pathology 2

 - POSTER SESSION

 - I ORAL SESSION - Disease Models 1

 - II ORAL SESSION - Disease Models 2

 - POSTER SESSION

SUNDAY September 22, 2002

MONDAY, September 23, 2002

I Oral Session - Mechanism of Activation

Co-chairs: Taroh Kinoshita – Francesco Tedesco

8:45am Endothelial cell proliferation induced by sublytic C5b-9  requires the activation of p70S6 kinase through a mechanism involving the G protein-dependent activity of phosphatidylinositol 3-kinase. (F. Niculescu, S .Hila, M. Fosbrink, L. Soane, T. Popescu, V. Shustova, V. Rus, M.L. Shin, H. Rus)

9:00am A novel mechanism for complement activation on the surface of B cells following antigen binding (A. Manderson, M. Botto, MJ. Walport, CC. Goodnow, CR. Parish)

9:15am Role of masp-1 and/or masp-3 in activation of the lectin pathway (M. Takahashi, M. Matsushita, Y. Endo, T. Fujita)

9:30am Endothelial cells express membrane-bound C7 that functions as a decoy acceptor for the assembling terminal complex (F. Bossi, V. Pellis, P. Macor, R. Bulla, R. Würzner, F. Tedesco)

9:45am The mannose-binding LECTIN-MASPs system: a potential link between complement and a defensive role of coagulation (K. Hajela, M. Kojima, J. Ferluga, G. Ambrus, P. Gal, R. B. Sim)

10:00am Pentraxin 3 can activate the classical complement pathway; a potential syngergistic role for PTX3 and complement in the clearance of apoptotic cells (A. J. Nauta, B. Bottazzi, A. Mantovani, W.J. Schwaeble, A.R. Gingras, S. Tzima, F. Vivanco, J. Egido, O. Tijsma, E.C. Hack, M.R. Daha, A. Roos)

II Oral Session - Complement Regulators

Co-chairs: Michael V. Holers – Anne Nicholson-Weller  

10:45am Functional characterization of human factor H-related protein 5 (FHR-5) reveals heparin binding and novel cofactor activity (T. G. Duthy, J.L. McRae, K.M. Griggs, B.F. Murphy, D.L. Gordon)

11:00am     Overcoming complement resistance of human carcinoma cells (N. Donin, K. Jurianz, L. Ziporen, S. Schultz, M. Kirschfink, Z. Fishelson )

11:15am Differential roles of daf, CRRY and CD59 in regulating alternative and classical pathway complement activation on murine erythrocytes (T. Miwa, L. Zhou, B. Hilliard, J.D. Lambris, H. Molina, W.C. Song)

11:30am     Characterization of membrane cofactor protein (MCP, CD46) on spermatozoa: role for a complement regulatory protein in fertilization (R.C. Riley, P. Tannenbaum, D. Abbott, J.P. Atkinson )

11:45am     Structural and functional characterisation of recombinant bovine conglutinin comprising the a-helical neck region and carbohydrate recognition domain (A. Prasad, R. Jinshan, A. Dodds, K.BM. Reid)

12:00noon     Localization of the host recognition functions of  complement factor H at the C-terminal: Implications for hemolytic uremic syndrome (M. K. Pangburn)  

12:15pm     Evolution of complement regulators: cloning and characterization of Ascidian rca candidates (S. Miyazawa, M. Nonaka)

Guest Lecture - CD1 Receptors in immune responses (Steven A. Porcelli. Albert Einstein College, New York, USA)

Poster Session (Sala Orione)

The Lectin Pathway

Mechanisms and Regulation of Activation

Structure and Function

Evolution and Ontogeny of Complement

TUESDAY, September 24, 2002

I Oral SessioN - Genetics and Structure

Co-chairs: David E. Isenman – Giacomo De Leo

8:30am     Structure, expression and genomic organization of six complement genes of an Ascidian, Ciona intestinalis (F. Yoshizaki, M. Nonaka)

8:45am     Duplicate genes for murine C1r and C1s generate alternative isomorphs in the liver and the male reproductive system (G. Garnier, A Circolo, Y. Xu, J.E. Volanakis)

9:00am     The crystal structure of human complement  protein C8g provides new insight into its function (J.M. Sodetz, E. Ortlund, C.L. Parker, S.F. Schreck, L. Lebioda)

9:15am     Solution structure and dynamics of central ccp module pair of decay accelerating factor (daf) (S. Uhrinova, F.Lin, D. Uhrin, G.Ball, M.E. Medof, P.N. Barlow)

9:30am     Residue Gln340, at the interface between the ccp1 and ccp2 modules of C1s, is a key element of C4 recognition (I. Bally, V. Rossi, N.M. Thielens, G.J. Arlaud)

9:45am     Crystal structure of the active catalytic domain of human C1r (M. Budayova-Spano, W. Grabarse, N.M. Thielens, H. Hillen,  M. Lacroix, M. Schmidt, J.C. Fontecilla-Camps, G.J. Arlaud,C.Gaboriaud)

10:00am     The interaction of isotypic forms of human C4b and C4d  fragments with complement receptor 1 as assessed by srface plasmon resonance (L. Clemenza, D.E. Isenman)

10:15am     Structural-activity-based design of compstatin using analogs nmr and combinatorial approaches (D.  Morikis, A. Soulika, J.D. Lambris)

II Oral Session - Immune Modulation

Co-chairs: John D. Lambris – Francesco Dieli

11:00am     Absence of the complement anaphylatoxin C3a receptor and the alternative pathway component Factor B suppresses th2 effector functions in a murine model of pulmonary allergy. (S. M. Drouin, D.B. Corry, J. Kildsgaard, T. J. Hollmann, R.A. Wetsel)

11:15am     Co-stimulation of T cells through complement regulatory proteins in kidney transplant rejection (H.P. Patel, J.R. Pratt, S.H. Sacks)

11:30am     CD46/CD3-activation of human cd4+ T cells induces  a T- regulatory 1 (TR1) phenotype (C. Kemper, A. C. Chan, J.M. Green, K.A. Brett, K.M. Murphy, J.P. Atkinson )

11:45am     Complement receptors CD21/CD35 are important in shaping the repertoire of natural IgM producing B-1 cells (M. Zhang, R. Reid, W. Austin, M. Xu, S. Woodlock, L. Kobzik, H.B. Hechtman, F.D. Moore, M. C. Carroll)

12:00noon     CR2 deficiency results in altered receptor  revision in peripheral B cells (S.  A. Boackle, K. K. Culhane, J. M. Brown, D. Nemazee, V. M. Holers)

12:15pm     Antigen localization on marginal zone macrophages (MZM) restores humoral immune response and IgG class switch in complement C4 deficient mice (B. Zachrau, K. Kropf, D. Finke, M.C. Carroll, M. Ma, H. Kirchner, S. Goerg)

12:30pm     A dispensable role for neutrophils, C5, and the classical  pathway of complement activation in the abnormal fetomaternal tolerance found in crry-deficient mice (D.  Mao, X. Wu, H. Molina)    

12:45pm     Reconstitution of C4-deficient mice with wild type bone marrow restores humoral immunity. (M. Gadjeva, A. Verschoor, M. Carroll)

WEDNESDAY, September 25, 2002

I Oral Session - Pathology 1

Co-chairs: Andrea J. Tenner – Ariella M. Rosengard

8:30am     The yeast and hyphal forms of Candida albicans bind complement alternative pathway regulators factor H and FHL-1 (T. Meri, A. Hartmann, D. Lenk, R. Eck, R. Würzner, J.Hellawage, S. Meri, P.F. Zipfel )

8:45am     Key role of the C5a receptor in E. Coli-induced oxidative burst and phagocytosis studied in a novel human whole blood model of inflammation (O.L. Brekke, H. Fure, D. Christiansen, K.T. Lappegård, M. Fung, J.D. Lambris, T. E. Mollnes)

9:00am     Characterization, enhancement and biological effects of the interaction of  mannose-binding lectin (mbl) with GP120 on HIV (G. T. Spear, M.L. Hart, M. Saifuddin)

9:15am     The streptococcal beta and pneumococcal hic proteins utilize homologous motifs for binding to the middle part of the complement inhibitor factor h (H. Jarva, T. Areschoug, J. Hellwage, M.J. Lehtinen, P.F. Zipfel,  G. Lindahl, S. Meri )

9:30am     Phosphoethanolamine substitutions on neisserial lipooligosaccharide are the preferred acceptors for C4 (S. Ram, A.D. Cox, U. Vogel, R. Boden, J. Plested, S. Meri, S. Getzlaff, JC Richards, ER Moxon, PA Rice )

9:45am     The presence of complement component C3 is important in generating a localized inflammatory response to cutaneous Staphylococcus aureus infection (D. L. Haviland, E. Brown, M. Hook, R. A. Wetsel)

10:00am     The interaction of C1 inhibitor with gram negative endotoxin (D. Liu, X. Gu, J. Scafidi, A.E. Davis)

II Oral Session - Pathology 2

Co-chairs: Rick A. Wetsel – Zvi Fishelson

10:45am     Complement C5 activation mediates antiphospholipid antibody-induced pregnancy loss (G. Girardi, J. Berman, L. Spruce, J.D. Lambris, M. Holers, J.E. Salmon)

11:00am     Factor H Gene Mutations In Hemolytic Uremic Syndrome: Reduced Protein Interactions Reveal A Role For Microvascular Damage And Thrombus Formation (T. Manuelian, J. Hellwage, S. Meri, J. Caprioli, M. Noris, Hartmut P.H. Neumann, G. Remuzzi, P. F. Zipfel )

11:15am     Complement C3a receptor: a newly identified innate immune endocrine regulatory pathway involved in control of inflammatory responses (K. Francis, B. M. Lewis, H. Akatsu, P. Monk, M. F. Scanlon, B.P. Morgan, J. Ham, P. Gasque)

11:30am     A role of glycated human CD59 and the complement system in the pathogenesis of chronic vascular complications of diabetes (X. Qin, N. Kumrei, A. Goldfine, L. Grubuissich, J. Halperin)

11:45am     Inefficient activation of T lymphocytes by gpi-anchor-negative cells: implications for paroxysmal nocturnal hemoglobinuria (T. Kinoshita, Y. Murakami, Y. Maeda, Jun-ichi Nishimura, N. Inoue)

12:00noon Proteomic analysis of mouse C3 deficiency and liver partial hepatectomy.(C.W. Strey, D. Spellman, M. Markieski and J.D. Lambris)

Guest Lecture - The various features of the HLA system

(E. Sergio Curtoni. University of Turin, Italy)

Poster Session - (Sala Orione)

Genetics and Regulation of Synthesis

Complement in Therapy

Complement as a diagnostic tool

Recombinant Complement proteins

Complement and Infection

THURSDAY, September 26, 2002

I Session - Disease Models 1

Co-chairs: Paul B. Morgan – Charlie J. Parker  

8:30am     Uncontrolled C3 activation causes membrano-proliferative glomerulonephritis in factor H-deficient mice (M. Botto, MC. Pickering, HT. Cook, J. Warren, AE Bygrave, J. Moss, MJ. Walport)

8:45am     Reconstitution of C1q-deficiency with bone marrow derived cells ameliorates the autommunity associated with C1q-deficiency (M. Botto, J. Cortes-Hernandez, L. Fossati-Jimack, S. Izui, F. Petry, M. Loos, MJ. Walport)

9:00am     Deletion of decay-accelerating factor (daf, CD55) exacerbates autoimmune disease development in MRL/lpr mice. (T. Miwa, M.A. Maldonado, L. Zhou, H.Y. Luo, V.P. Werth, M.P. Madaio, R.A. Eisenberg, W.C. Song)

9:15am     Complement inhibition with crry prevents development of lupus nephritis (L. Bao, J. Rakstang, V.M. Holers, R. J. Quigg)

9:30am     Protective effects by C5aR antagonist (C5ara) during experimental sepsis (M. Huber-Lang, V. Sarma, E. Younkin, S.R. McGuire, KT Lu, TA Neff, RF Guo, F. Gebhard, J.Lambris, PA Ward)

9:45am     Reversal of the increased vascular permeability in C1 inhibitor deficient mice: Therapeutic approaches (E. D. Han, E. Pappalardo, J. Scafidi, A. E. Davis)

10:00am     Effect of complement factor D deficiency on renal disease in MRL/lpr mice (M. Elliott, P. Ruiz, Y. Ma, G. Gilkeson)

10:15am     Murine CD93 (C1qRP) deficiency causes a defect in apoptotic cell clearance in vivo (PJ. Norsworthy, L. Fossati-Jimack, J. Cortes-Hernandez, P. Taylor, A. Bygrave, MJ Walport, M. Botto)

II Oral Session - Disease Models 2

Co-chairs: Luigi Bergamaschini – Mohamed R. Daha

11:00am     Complement inhibition ameliorates disease in lupus cerebritis (J J. Alexander, L. Bao, V. M. Holers, R. J. Quigg)

11:15am     Complement is not essential for the development of neuritic (senile) plaques in an animal model of Alzheimer’s disease.(N. Cooper, B.M. Bradt, K. Hsiao, M.C. Carroll, J.X. Yu)

11:30am     Mechanism for C1-inhibitor neuroprotection in an animal model of transient focal brain ischemia (M. G. De Simoni, C. Storini, E. Rossi, A. Agostoni, R. Veerhuis, L. Bergamaschini)                     

11:45am     The effect of terminal complement complex on apoptosis gene expression in experimental autoimmune encephalomyelitis (H. Rus, S. Weerth, L. Soane, F. Niculescu, V. Rus, C. Raine, M.L. Shin)

12:00noon     CD59-deficiency exacerbates clinical disease and central nervous system inflammation in murine experimental allergic encephalomyelitis (R. J. Mead, J. W. Neal, B. P. Morgan)

12:15pm     The role of complement in liver regeneration (C. W. Strey, M. Markiewski, D. Mastellos, R. Tudoran, L. Greenbaum, J. D. Lambris)

12:30pm     A non-transgenic strategy for tissue engineering for the modulation of transplant ischemia/reperfusion injury. (M. Jones, J.R. Pratt, I. Dodd, R.A.G. Smith, S.H. Sacks)

12:45pm     Decay accelerating factor deficiency increases susceptibility to dextran sulfate sodium-induced colitis in mice (F. Lin, D. Spencer, A. D. Levine, M. E. Medof)

Poster Session - (Sala Orione)

Complement and Apoptosis

Immune Regulation

Animal Models

Human Pathology