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Proteomic analysis of Parietaria judaica pollen and allergen profiling by an immunoproteomic approach

  • Authors: Barranca, M.; Fontana, S.; Taverna, S.; Duro, G.; Zanella Cleon, I.; Becchi, M.; DE LEO, G.; Alessandro, R.
  • Publication year: 2010
  • Type: Articolo in rivista (Articolo in rivista)
  • Key words: allergens, Parietaria judaica, immunoproteome, pollen
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Parietaria judaica pollen is a common cause of airway allergic disease in the Mediterranean area. Proteome analysis of mature Parietaria judaica pollen by two-dimensional gel electrophoresis (2-DE) and mass spectrometry has established the first reference proteome map of this weed. Proteins involved in a variety of cellular functions as well as the occurrence of allergens were detected. By using 2-DE and immunoblotting with sera from Parietaria judaica allergic patients we obtained a more detailed characterization of Parietaria judaica allergen profile