The effects of dexamethasone on human synovial fibroblast-like cells, from ostheoarthritic joints, in culture
- Authors: R. VENTO, TORREGROSSA M.V., M. GIULIANO, G. GRECOMORO, F. PICCIONE
- Publication year: 1990
- Type: Articolo in rivista (Articolo in rivista)
- OA Link: http://hdl.handle.net/10447/62888
Abstract
The effect of Dexamethasone (DEX) on cell division and macromolecular synthesis was investigated in a line (Mc Coy cells, A 9) of synovial fibroblast-like cells derived from human osteoarthritic joints. DEX markedly reduced the proliferation of Mc Coy cells in a time and dose-dependent manner. The maxiamal inhibition (45 %) was found at 500 nM DEX 24 h after incubation and was accompanied by the appearance of giant macrophage-like cells. After DEX treatment cells showed increased content of DNA, proteins and RNA together with the reduction of [3H]-thymidine incorporation into the TCA-precipitable fraction.