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GIUSEPPINA CAPRA

Detection of Human Papillomavirus DNA in cervical samples: analysis of the new PGMY-PCR compared to Hybrid Capture II, MY-PCR and a Two-step nested PCR assay.

  • Authors: GIOVANNELLI, L; A LAMA; CAPRA, G; GIORDANO, V; ARICO, P; AMMATUNA, P
  • Publication year: 2004
  • Type: Articolo in rivista (Articolo in rivista)
  • Key words: Papillomavirus Infections; Tumor Virus Infections; Polymerase Chain Reaction
  • OA Link: http://hdl.handle.net/10447/9831

Abstract

The PGMY-PCR for human papillomavirus (HPV) was evaluated, in parallel with nested PCR (nPCR), in samples with noted Hybrid Capture II (HCII) and MY-PCR results. PGMY-PCR detected HPV DNA in 2.5% of HCII-negative-MY-PCR-negative samples and in 71.7% of HCII-positive-MY-PCR-negative samples; also, it detected the MY-PCR-negative-nPCR-negative types HPV-42, HPV-44, HPV-51, HPV-87, and HPV-89.