High-Performance Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry (HPLC-ESI-MSn) Analysis and Bioactivity Useful for Prevention of “Diabesity” of Allium commutatum Guss
- Authors: Loizzo M.R.; Tundis R.; Sut S.; Dall'Acqua S.; Ilardi V.; Leporini M.; Falco T.; Sicari V.; Bruno M.
- Publication year: 2020
- Type: Articolo in rivista
- Key words: Aerial parts; Antioxidant activity; Bulbs; Carbohydrate-hydrolysing enzymes inhibition; Garlic; Lipase inhibition; Antioxidants; Chromatography, High Pressure Liquid; Enzyme Inhibitors; Italy; Plant Extracts; Allium; Tandem Mass Spectrometry
- OA Link: http://hdl.handle.net/10447/454604
Abstract
This study aimed at evaluating and comparing the chemical profile obtained by HPLC-ESI-MSn analysis, the inhibitory activity of enzymes linked to obesity (α-amylase, α-glucosidase, and lipase) and the antioxidant properties (DPPH, ABTS, FRAP, and β-carotene bleaching tests) of ethanol extracts of bulbs (BE) and aerial parts (APE) from Allium commutatum Guss. (known in Italy as “aglio delle isole”). The chemical profile revealed alliin as the main abundant compound with values of 31.5 and 38.8 mg/g extract for BE and APE, respectively. APE is rich also in quercetin (38.5 mg/g extract) and luteolin (31.8 mg/g extract). Bulbs extract exhibited the highest activity as inhibitor of enzymes linked to obesity. Except for DPPH test, APE showed the highest antioxidant potential with IC50 of 7.6 and 56.6 μg/mL in ABTS and β-carotene bleaching test after 60 min of incubation, respectively. In conclusion, the present investigation revealed A. commutatum bulbs and aerial parts as a promising source of inhibitors of enzyme linked to the obesity and of antioxidant compounds.