Optimization of clustering parameters for single-cell RNA analysis using intrinsic goodness metrics

Abstract

The accurate clustering of cell subpopulations is a crucial aspect of single-cell RNA sequencing. The ability to correctly subdivide cell subpopulations hinges on the efficacy of unsupervised clustering. Despite the advancements and numerous adaptations of clustering algorithms, the correct clustering of cells remains a challenging endeavor that is dependent on the data in question and on the parameters selected for the clustering process. In this context, the present study aimed to predict the accuracy of clustering methods when varying different parameters by exploiting the intrinsic goodness metrics.