Molecular and taxonomic characterization of a endophytic fungus isolated from Helleborus bocconei subsp. intermedius (Ranunculaceae)
- Autori: Spadaro,V;Raimondo,FM;Pasqualetti,M
- Anno di pubblicazione: 2014
- Tipologia: Articolo in rivista (Articolo in rivista)
- Parole Chiave: Chaetomium strumarium, Chaetomiaceae, endophyte, endemic plant, molecular analysis, folk veterinary medicine.
- OA Link: http://hdl.handle.net/10447/101945
Abstract
A non-sporulating fungus was isolated from different organs of Helleborus bocconei subsp. intermedius (Ranunculaceae) endemic to southern Italy and Sicily, known for the traditional use of dried roots in the treatment of lung diseases of cattle and horses. Molecular characterization of endophytic fungus based on the internal transcribed spacer (ITS) region of the rRNA gene sequences was done. The DNA sequence of full length ITS region of the studied fungus was a 100% match to that of Chaetomium strumarium strain dH 21642 (GenBank accession number JX280851.1). The morphological characters of colony and mycelium of this microfungus are reported here.