Analysis of HLA-DRB1, DQA1, DQB1 haplotypes in Sardinian centenarians.

Abstract

Some genetic determinants of longevity might reside in those polymorphisms for the immune system genes that regulate immune responses. Many longevity association studies focused their attention on HLA (the human MHC)polymorphisms, but discordant results have been obtained. Sardinians are a relatively isolate population and represent a suitable population for association studies. Some HLA-DR and DQ alleles form very stable haplotypes with a strong linkage disequilibrium. In a previous study on Sardinian centenarians we have suggested that HLADRB1* 15 allele might be marginally associated to longevity. HLA-DR,DQ haplotypes are in strong linkage disequilibrium and well conserved playing a role in the association to diseases. Hence, we have evaluated, by amplification refractory mutation system/polymerase chain reaction (ARMS-PCR) the HLADQA1 and HLA-DQB1 allele frequencies in 123 centenarians and 92 controls from Sardinia to assess whether the association to HLADRB1* 15 allele may be due to the other genes involved in the HLA-DR,DQ haplotypes. The frequencies of HLADQA1, DQB1 haplotypes were not significantly modified in centenarians. Nevertheless by evaluating the frequency of DRB1*15 linked haplotypes, we observed a not significant increase in centenarians of HLA-DQA1*01,DQB1*05 and HLA-DQA1*01,DQB1*06 haplotypes. These data suggest that these haplotypes might have a role in determining life span expectancy and longevity.