A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting
- Autori: Palumbo, F.; BAVUSO VOLPE, A.; Bongiovì, F.; Pitarresi, G.; Giammona, G.
- Anno di pubblicazione: 2015
- Tipologia: Articolo in rivista (Articolo in rivista)
- Parole Chiave: ATRP; CD44; hyaluronic acid; siRNA delivery; tumor targeting; Antigens, CD44; Cell Line, Tumor; Drug Delivery Systems; Humans; Methacrylates; Neoplasm Proteins; Genetic Vectors; Hyaluronic Acid; Neoplasms; RNA, Small Interfering; Biotechnology; Bioengineering; Biomaterials; Polymers and Plastics; Materials Chemistry2506 Metals and Alloys
Two derivatives of hyaluronic acid (HA) have been synthesized by atom transfer radical polymerization (ATRP), starting from an ethylenediamino HA derivative (HA-EDA) and by using diethylaminoethyl methacrylate (DEAEMA) as a monomer for polymerization. Both samples, indicated as HA-EDA-pDEAEMA a and b, are able to condense siRNA, as determined by gel retardation assay and resulting complexes show a size and a zeta potential value dependent on polymerization number, as determined by dynamic light scattering measurements. In vitro studies performed on HCT 116 cell line, that over express CD44 receptor, demonstrate a receptor mediated uptake of complexes, regardless of their surface charge. New cationic HA derivatives (HA-EDA-pDEAEMA), synthesized by atom transfer radical polymerization (ATRP) are able to complex siRNA and to form self-assembled nanosystems. In vitro studies performed on HCT 116 cell line, which over express CD44 receptor, demonstrate a receptor-mediated uptake of complexes.