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Uptake of silica covered Quantum Dots into living cells: Long term vitality and morphology study on hyaluronic acid biomaterials

  • Autori: D'Amico, M.; Fiorica, C.; Palumbo, F.; Militello, V.; Leone, M.; Dubertret, B.; Pitarresi, G.; Giammona, G.
  • Anno di pubblicazione: 2016
  • Tipologia: Articolo in rivista (Articolo in rivista)
  • Parole Chiave: Chondrocyte bovine cells; Fluorescence confocal microscopy; Hyaluronic acid; Labelling; Long term cell staining; Silica Quantum Dots; Animals; Biocompatible Materials; Cattle; Cell Shape; Cell Survival; Chondrocytes; Hyaluronic Acid; Microscopy, Fluorescence; Nanoparticles; Quantum Dots; Silicon Dioxide; Materials Science (all); Condensed Matter Physics; Mechanics of Materials; Mechanical Engineering
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Quantum Dots (QDs) are promising very bright and stable fluorescent probes for optical studies in the biological field but water solubility and possible metal bio-contamination need to be addressed. In this work, a simple silica-QD hybrid system is prepared and the uptake in bovine chondrocytes living cells without any functionalization of the external protective silica shield is demonstrated. Moreover, long term treated cells vitality (up to 14 days) and the transfer of silica-QDs to the next cell generations are here reported. Confocal fluorescence microscopy was also used to determine the morphology of the so labelled cells and the relative silica-QDs distribution. Finally, we employ silica-QD stained chondrocytes to characterize, as proof of concept, hydrogels obtained from an amphiphilic derivative of hyaluronic acid (HA-EDA-C 18) functionalized with different amounts of the RGD peptide.