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SIMONA FONTANA

Production of an egg yolk antibody against Parietaria judaica 2 allergen.

  • Authors: Alessandro, R.; Gallo, A.; Barranca, M.; Principe, S.; Taverna, S.; Duro, G.; Cassata, G.; Becchi, M.; Fontana, S.; DE LEO, G.
  • Publication year: 2009
  • Type: Articolo in rivista (Articolo in rivista)
  • Key words: Parietaria judaica; egg yolk antibody; allergen
  • OA Link: http://hdl.handle.net/10447/37440

Abstract

Specific antibodies are essential tools for studying proteins as well as for diagnostic research in biomedicine. The egg yolk of immunized chicken is an inexpensive source of high-quality polyclonal antibodies. The 12-kDa Parietaria judaica 2 allergen was expressed as a fusion protein and was used to immunize Leghorn chickens. In this paper, we show, using 2-dimensional gel electrophoresis and immunoblotting, that chicken antibodies raised against a recombinant allergen can be used to recognize similar proteins from a pollen raw extract. Allergen identity was confirmed by nanoLC-nanospray-tandem mass spectrometry analysis. Our data demonstrate for the first time that a synergistic combination of molecular biology, 2-dimensional PAGE, and use of nonmammalian antibodies represents a powerful tool for reliable identification of allergens.