Salta al contenuto principale
Passa alla visualizzazione normale.

MATTEO CAMMARATA

Cloning and expression of a type IX-like collagen in tissues of the ascidian Ciona intestinalis

  • Autori: Vizzini, A; Arizza, V; Cervello, M; Cammarata, M; Gambino, R; Parrinello, N
  • Anno di pubblicazione: 2002
  • Tipologia: Articolo in rivista (Articolo in rivista)
  • Parole Chiave: cDNA library; Ascidian; Type IX-like collagen; mRNA localization
  • OA Link: http://hdl.handle.net/10447/65052

Abstract

Collagens are highly preserved proteins in invertebrates and vertebrates. To identify the collagens in urochordates, the total RNA extracted from the pharynx of the ascidian Ciona intestinalis was hybridized with a heterologous probe specific for the echinoderm Paracentrotus lividus fibrillar type I-like larval collagen. Using this probe, two main bands (i.e. 6 and 2.8 kb mRNA) were observed on Northern blot hybridization. The cDNA library prepared from poly(A) +RNA extracted from pharyngeal tissue was screened and a cDNA that specifies a type IX-like collagen was identified. This molecule presents a conceptual open reading frame for a protein containing 734 amino acids. In particular, we showed a 1a chain type IX-like collagen characterized by three short triple-helical domains interspersed with four non-triple-helical sequences, with structural features of fibril-associated collagens with interrupted triple-helices (FACIT) collagens. Northern blot hybridizations indicate a 2.8 kb transcript size. Sequence comparison indicated homology (47.64%, 48.95%) between the type IX-like collagen of C. intestinalis and mouse and human type IX collagen. In situ hybridization of tunic and pharynx tissues shows the presence of transcripts in connective tissue cells.