Ruta chalepensis L. (Rutaceae) leaf extract: chemical composition, antioxidant and hypoglicaemic activities
- Autori: Loizzo, M; Falco, T; Bonesi, M; Sicari, V; Tundis, R; Bruno, M
- Anno di pubblicazione: 2018
- Tipologia: Articolo in rivista (Articolo in rivista)
- Parole Chiave: HPLC analysis; Ruta chalepensis; antioxidant activity; hypoglycaemic activity; leaves; Antioxidants; Biphenyl Compounds; Dose-Response Relationship, Drug; Enzyme Inhibitors; Glycoside Hydrolase Inhibitors; Hesperidin; Hydrolysis; Inhibitory Concentration 50; Lipid Peroxidation; Picrates; Plant Extracts; Plant Leaves; Ruta; Rutin; alpha-Amylases; beta Carotene
- OA Link: http://hdl.handle.net/10447/300285
Abstract
Ruta chalepensis L. (Rutaceae) leaf extract was investigated for its chemical profile and antioxidant and hypoglycaemic properties. The antioxidant effects were investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH), Chi-carotene bleaching, and metal chelating activity assays. The carbohydrate-hydrolysing enzymes inhibition assay was used to test the hypoglycaemic potential. R. chalepensis showed a high content of hesperidin and rutin with values of 591.9 and 266.7 mg/g dry extract, respectively. The extract exhibited a promising protection of lipid peroxidation (IC50 value of 16.9 mu g/mL) and inhibited both alpha-amylase and alpha-glucosidase enzymes in a concentration-dependent manner. The highest activity was found against alpha-amylase (IC50 value of 69.0 mu g/mL). The obtained results support the use of R. chalepensis leaves as healthy food ingredients.