Pulsed field gel electrophoresis and genome size estimates
- Authors: Alduina, R.; Pisciotta, A.
- Publication year: 2015
- Type: Capitolo o Saggio (Capitolo o saggio)
- Key words: Genome size; Genome topology; Megaplasmids; Multi-replicons; Archaea; Bacteria; Base Composition; Chromosomes, Archaeal; Chromosomes, Bacterial; DNA, Archaeal; DNA, Bacterial; Electrophoresis, Agar Gel; Electrophoresis, Gel, Pulsed-Field; Physical Chromosome Mapping; Plasmids; Genome Size; Genome, Archaeal; Genome, Bacterial; Molecular Biology; Genetics; Medicine (all)
- OA Link: http://hdl.handle.net/10447/253753
Abstract
Pulsed field gel electrophoresis (PFGE) is a quick and reliable procedure to resolve DNA molecules larger than 30 kb by applying an electric field that periodically changes direction. This technique can be used to estimate genome size of a microorganism, to reveal if a genome is circular or linear, to indicate the presence of megaplasmids, and to show if a strain contains only one or more chromosomes.