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RICCARDO ALESSANDRO

Production of an egg yolk antibody against Parietaria judaica 2 allergen.

  • Autori: Alessandro, R; Gallo, A; Barranca, M; Principe, S; Taverna, S; Duro, G; Cassata, G; Becchi, M; Fontana, S; De Leo, G
  • Anno di pubblicazione: 2009
  • Tipologia: Articolo in rivista (Articolo in rivista)
  • Parole Chiave: Parietaria judaica; egg yolk antibody; allergen
  • OA Link: http://hdl.handle.net/10447/37440

Abstract

Specific antibodies are essential tools for studying proteins as well as for diagnostic research in biomedicine. The egg yolk of immunized chicken is an inexpensive source of high-quality polyclonal antibodies. The 12-kDa Parietaria judaica 2 allergen was expressed as a fusion protein and was used to immunize Leghorn chickens. In this paper, we show, using 2-dimensional gel electrophoresis and immunoblotting, that chicken antibodies raised against a recombinant allergen can be used to recognize similar proteins from a pollen raw extract. Allergen identity was confirmed by nanoLC-nanospray-tandem mass spectrometry analysis. Our data demonstrate for the first time that a synergistic combination of molecular biology, 2-dimensional PAGE, and use of nonmammalian antibodies represents a powerful tool for reliable identification of allergens.